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Since sperm cells are transcriptionally inert and rely on interactions with epididymosomes (epEVs) to acquire molecules, herein, we investigated the effects of the addition of epEVs to the cryopreservation extender on post\u2010thaw sperm quality and fertility potential. Epididymal sperm were obtained from six bulls (\n                    <jats:italic>n\u2009<\/jats:italic>\n                    =\u20096) and a pool of epEVs was formed from the epididymal fluid of 15 bulls. After, a total of 30\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    sperm was cryopreserved in the presence (epEVs) or not (Control) of PKH67 green\u2010labeled epEVs. Post\u2010thaw, sperm were evaluated for interaction with epEVs by flow cytometry (FC) and fluorescence microscopy (FM); motility, vigor, and morphology by microscopy; membrane integrity by FC and FM; microRNA levels by qPCR; and fertility potential by in vitro embryo production (IVEP). EpEVs\u2010sperm showed interaction with green\u2010epEVs, and higher motility and plasma membrane integrity compared to Control\u2010sperm. Levels of 28 microRNAs were higher or uniquely detected in epEVs\u2010sperm. IVEP with epEVs\u2010sperm resulted in a higher rate of hatched blastocyst. Thus, epEVs\u2010supplemented to the sperm cryopreservation extender improved post\u2010thaw sperm quality and embryo developmental potential. These findings provide new insights into the molecular modulation of sperm fertility.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70138","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:03:11Z","timestamp":1785398591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epididymosome\u2010Supplemented Extender Induces Changes in Morpho\u2010Functional Traits and microRNA Levels of Post\u2010Thaw Sperm and Improves Embryo Developmental Potential"],"prefix":"10.1002","volume":"93","author":[{"given":"Laura Gabrielli","family":"Haupenthal","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alice","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cibele Maria","family":"Prado","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Nespolo","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela Melendes","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Maria da Silva","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio Vieira","family":"Meirelles","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliano Coelho","family":"da Silveira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering University of S\u00e3o Paulo Pirassununga S\u00e3o Paulo 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