{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:19:41Z","timestamp":1787757581717,"version":"build-2784847793"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV\u2010003768"],"award-info":[{"award-number":["INV\u2010003768"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Zinc deficiency is associated with impaired male fertility and with reduced DNA and histone methylation in oocytes, but the temporal relationship between acute dietary zinc deficiency and sperm function is unclear. To fill in these gaps, the effects of acute dietary zinc deficiency on sperm function were tested. Five\u2010week\u2010old F1 (DBA\u2009\u00d7\u2009C57BL\/6) male and female mice were fed a control diet (29\u2009mg Zn\/kg) or zinc\u2010deficient diet (&lt;\u20091\u2009mg Zn\/kg) for 6 days (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200911\/group), a duration selected to model acute zinc depletion based on previous studies demonstrating rapid reproductive defects within a similar timeframe (Tian and Diaz\u00a02012). Sperm number and motility were evaluated by computer\u2010assisted sperm analysis in all animals (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200911), and testicular histology and DDX4 and YBX2 (MSY2) immunostaining and global m6A levels in testes and liver were measured in a subset of males (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) and in ovaries and liver in females (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) by an ELISA\u2010based assay. Zinc deficiency did not alter body weight but significantly reduced total sperm number recovered from the cauda epididymis and impaired total and progressive motility, straight\u2010line velocity, and hyperactivation. Testes from zinc\u2010deficient males showed reduced thickness of germinal epithelium and increased basement membrane thickness, disorganized seminiferous tubules, and fewer DDX4\u2010positive germ cells, while YBX2\u2010positive cells were largely preserved. Despite these structural and functional defects, zinc deficiency did not significantly change global m6A levels in testes or ovaries, although hepatic m6A methylation was increased in females. These findings demonstrate that a short\u2010term zinc\u2010deficient diet rapidly compromises sperm production, motility, and testicular morphology without measurably altering global gonadal mRNA methylation.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70144","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:45:47Z","timestamp":1787553947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acute Dietary Zinc Deficiency Impairs Sperm Motility but Not Gonadal mRNA Methylation in Mice"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5941-8810","authenticated-orcid":false,"given":"Ngozi O.","family":"Ibadin","sequence":"first","affiliation":[{"name":"Integrative and Biomedical Physiology Graduate Program, Department of Animal Science, Huck Institute of Life Sciences Pennsylvania State University University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2875-7393","authenticated-orcid":false,"given":"Francisco J.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Integrative and Biomedical Physiology Graduate Program, Department of Animal Science, Huck Institute of Life Sciences Pennsylvania State University University Park Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207796"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14647273.2018.1494390"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaab020"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.110.131318"},{"key":"e_1_2_10_6_1","first-page":"69","article-title":"Zinc Is an Essential Element for Male Fertility: A Review of Zn Roles in Men's Health, Germination, Sperm Quality, and Fertilization","volume":"19","author":"Fallah A.","year":"2018","journal-title":"Journal of Reproduction & Infertility"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab023"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09597-4_14"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03823-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485019708994883"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.091629"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-024-04441-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04523-y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1902110"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00214.2022"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1010087"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2023.0410"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-1591.139570"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2012.12.014"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes16080951"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-022-03324-1"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-016-0841-x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.113910"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1599"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.01.015"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-025-04615-z"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-024-05536-1"},{"key":"e_1_2_10_28_1","volume-title":"The Induction of Sperm\u2010Shape Abnormalities in Mice and Humans","author":"Wyrobek A. 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