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During this process, male germ cells are interconnected by intercellular bridges (ICBs). Although ICB fragmentation has been implicated in the replenishment of the SSC pool, the molecular mechanisms underlying this process remain poorly understood. Our previous study demonstrated that JMJD3 deficiency promotes spermatogonial fragmentation and preserves spermatogenesis during ageing. To investigate the molecular basis underlying these phenotypes, we performed H3K27me3 ChIP\u2010seq using a spermatogonia\u2010enriched CD9\u2010positive cell fraction together with RNA\u2010seq of SSC\u2010enriched spermatogonial populations from JMJD3\u2010deficient mice. Cross\u2010dataset comparison revealed extensive alterations in H3K27me3 landscapes accompanied by age\u2010 and JMJD3\u2010dependent transcriptional changes. RNA\u2010seq demonstrated downregulation of multiple signaling pathways, including NF\u2010\u03baB and TNF signaling, together with dysregulation of extracellular matrix (ECM)\u2010 and cell adhesion\u2010related genes in JMJD3\u2010deficient spermatogonia. Consistent with these transcriptional changes, JMJD3\u2010deficient spermatogonia exhibited excessive ECM accumulation and altered expression of adhesion\u2010associated genes in vivo. Although several mesenchymal\u2010associated genes were upregulated, canonical cadherin switching was not observed, indicating the presence of partial epithelial\u2010mesenchymal transition (EMT)\u2010like transcriptional features rather than a complete EMT program. Cross\u2010dataset comparison further identified Ptk2b as a candidate gene associated with JMJD3\u2010dependent H3K27me3 changes. These findings suggest that JMJD3 deficiency is associated with extensive epigenomic remodeling and adhesion\u2010related transcriptional alterations in aged spermatogonia and provide candidate molecular pathways that may contribute to the maintenance of spermatogenesis during ageing.<\/jats:p>","DOI":"10.1002\/mrd.70147","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T16:09:57Z","timestamp":1789056597000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["JMJD3 Deficiency Alters H3K27me3 Landscapes and Adhesion\u2010Related Transcriptional Programs in Aged Spermatogonia"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9317-5515","authenticated-orcid":false,"given":"Yusuke","family":"Sakurai","sequence":"first","affiliation":[{"name":"Laboratory of Zoology, Graduate School of Bioresource and Bioenvironment Sciences Kyushu University Fukuoka 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