{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:52:18Z","timestamp":1791129138844,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312742\/2022\u20103"],"award-info":[{"award-number":["312742\/2022\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["405048\/2024\u20106"],"award-info":[{"award-number":["405048\/2024\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["445245\/2024\u20107"],"award-info":[{"award-number":["445245\/2024\u20107"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["448945\/2025\u20108"],"award-info":[{"award-number":["448945\/2025\u20108"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005283","name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Reproduction Devel"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to characterize the proteome of sperm and seminal plasma (SP) from rams with high semen freezability (HF) and low semen freezability (LF). Eleven ejaculates were collected from 12 rams, at 3\u2010day intervals, and cryopreserved (132 semen samples). For proteomic analysis, sperm and SP samples were pooled separately for each ram. Pools 1 and 2 included ejaculates obtained on odd\u2010numbered and even\u2010numbered collection days, respectively. This generated 24 sperm and 24 SP pools. Sperm and SP proteins were analyzed by mass spectrometry, and computational biology platforms, used for calculation of differentially abundant proteins (DAPs;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) in HF (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) and LF (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20097) rams. Total sperm motility decreased from 91.87%\u2009\u00b1\u20090.63% in fresh to 65.65%\u2009\u00b1\u20092.58% in frozen\u2010thawed semen of HF rams; and decreased from 89.47%\u2009\u00b1\u20091.15% to 50.05%\u2009\u00b1\u20092.33% in LF rams. Reduction in total sperm motility after cryopreservation was lower in HF (26.22%\u2009\u00b1\u20092.39%) compared to LF rams (37.70%\u2009\u00b1\u20092.23%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.006). Sperm progressive motility decreased less in HF rams (from 87.20%\u2009\u00b1\u20090.50% to 58.12%\u2009\u00b1\u20092.82%) than in LF rams (from 85.54%\u2009\u00b1\u20091.41% to 44.00\u2009\u00b1\u20092.13;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.006). Rams with the HF phenotype had better plasma membrane (39.00%\u2009\u00b1\u20092.79% vs. 15.14%\u2009\u00b1\u20090.92%) and acrosome integrity (37.54%\u2009\u00b1\u20092.72% vs. 15.40%\u2009\u00b1\u20091.92%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) than LF rams. Sperm motility was similar in fresh semen of HF and LF rams. There were 1599 and 1677 sperm proteins identified in HF and LF rams, with 73 and 76 DAPs, respectively. SP had 1127 proteins, with 25 and 45 DAPs in HF and LF rams. This is the greatest coverage ever published about the ram SP proteome. Sperm proteins with greater abundance in HF rams were extracellular and immune\u2010related proteins, antiproteases, chaperones and structural regulators, contributing to cytoskeletal integrity, apoptotic resistance and maintenance of proteostasis. Sperm proteins with greater abundance in LF rams were mainly mitochondrial subunits, axonemal components, and bioenergetic enzymes, indicating greater reliance on oxidative processes. Major components of HF SP participate in sperm protection, stabilization of membrane lipids, calcium fluxes, epididymal maturation and ATP availability. The major LF SP proteome was associated with cellular stress, inflammatory responses, oxidative damage and ATP production. Thus, the nature of sperm and SP proteome atlas potentially defines ram sperm cryoresilience.\n                  <\/jats:p>","DOI":"10.1002\/mrd.70148","type":"journal-article","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:16:17Z","timestamp":1791126977000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Global Proteomic Analysis of Sperm and Seminal Plasma From Tropically\u2010Adapted Rams With Contrasting Parameters of Frozen\u2010Thawed Semen"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5482-3446","authenticated-orcid":false,"given":"Wallisson Bruno de Morais","family":"Pacheco","sequence":"first","affiliation":[{"name":"Graduate Program in Veterinary Sciences, School of Veterinary Medicine Cear\u00e1 State University Fortaleza 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elisabete Amaral","family":"Moraes","sequence":"additional","affiliation":[{"name":"Drug Research and Development Center, School of Medicine Federal University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5255-4644","authenticated-orcid":false,"given":"Carlos Roberto Koscky","family":"Paier","sequence":"additional","affiliation":[{"name":"Drug Research and Development Center, School of Medicine Federal University of Cear\u00e1 Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3606-6808","authenticated-orcid":false,"given":"Ana Cristina de Oliveira","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Center of Experimental Biology The University of Fortaleza (Unifor) Fortaleza 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