{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:54:14Z","timestamp":1761101654809},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,11,21]],"date-time":"2003-11-21T00:00:00Z","timestamp":1069372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The preferential accumulation of sickle blood cells in tumor vasculature is demonstrated noninvasively using MRI and sickle red blood cells loaded with Gd\u2010DTPA and invasively by two other techniques. The distribution of red blood cells in rat brain tumors relative to normal brains were measured using three separate techniques: MRI of Gd\u2010DTPA loaded cells, fluorescent microscopy detection of Oregon Green 488 fluorescence conjugated to a streptavidin\u2010biotin complex that binds to red blood cell surface proteins, and autoradiography using a technetium <jats:sup>99m<\/jats:sup>Tc\u2010labeling kit. Labeled red cells were infused intravenously in rats with brain tumors. Sickle cells preferentially accumulated in tumor relative to normal brain, with highest concentrations near the tumor \/ normal tissue boundary, whereas control normal red cells did not preferentially aggregate at the tumor periphery. This demonstrates the potential of sickle red blood cells to accumulate in the abnormal tumor vessel network, and the ability to detect their aggregation noninvasively and at high spatial resolution using MRI. The application of the noninvasive measurement of sickle cells for imaging tumor neovasculature, or as a delivery tool for therapy, requires further study. Magn Reson Med 50:1209\u20131214, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrm.10646","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T14:18:19Z","timestamp":1070374699000},"page":"1209-1214","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Sickle red blood cells accumulate in tumor"],"prefix":"10.1002","volume":"50","author":[{"given":"S.L.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.R.","family":"Ewing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.N.","family":"Nagaraja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.S.","family":"Swerdlow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.D.","family":"Fenstermacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.H.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,11,21]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0195-27"},{"key":"e_1_2_7_3_2","first-page":"6033","article-title":"Arsenic trioxide causes selective necrosis in solid murine tumors by vascular shut down","volume":"59","author":"Lew YS","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/02656738609012389"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(99)00273-4"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910400118"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.56.5.548"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.24.9868"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.20.9612"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(85)90217-2"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90038-6"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002619900393"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)63707-8"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199501000-00001"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.2001.6426"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(76)90025-X"},{"key":"e_1_2_7_17_2","first-page":"330","article-title":"Diameter of myocardial capillaries","volume":"4","author":"Sobin S","year":"1972","journal-title":"Microvasc Res"},{"key":"e_1_2_7_18_2","first-page":"H725","article-title":"Variability in erythrocyte deformability among various mammals","volume":"236","author":"Smith JE","year":"1978","journal-title":"Am J Physiol"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/028418699432536"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1053\/crad.2000.0442"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1259\/bjr.73.867.10817037"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0598-623"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/73780"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00069-7"}],"container-title":["Magnetic Resonance in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrm.10646","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrm.10646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T14:32:01Z","timestamp":1697207521000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrm.10646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11,21]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1002\/mrm.10646"],"URL":"https:\/\/doi.org\/10.1002\/mrm.10646","archive":["Portico"],"relation":{},"ISSN":["0740-3194","1522-2594"],"issn-type":[{"value":"0740-3194","type":"print"},{"value":"1522-2594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11,21]]},"assertion":[{"value":"2002-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-08-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-11-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}