{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:09:55Z","timestamp":1792462195531,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,18]],"date-time":"2005-11-18T00:00:00Z","timestamp":1132272000000},"content-version":"vor","delay-in-days":5831,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[1989,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Alterations in cytosolic metabolites and [Mg<jats:sup>2+<\/jats:sup>], were monitored using <jats:sup>31<\/jats:sup>P magnetic resonance spectroscopy during inotropic stimulation of isolated rat heart [10 n <jats:italic>M<\/jats:italic> isoproterenol, 0.6 \u03bc<jats:italic>M<\/jats:italic> isobutyl\u20101\u2010methylxanthine (IBMX), 5 \u03bc<jats:italic>M<\/jats:italic> ouabain]. All drugs significantly elevated contractile function (rate\u2010pressure product) and <jats:italic>MVO<jats:sub>2<\/jats:sub><\/jats:italic> by approximately 100\u2010150% (<jats:italic>P<\/jats:italic> 0.001), decreased cytosolic [creatine phosphate] ([CrP]) and [ATP] (approximately 65 and 80% of control values, respectively) (<jats:italic>P<\/jats:italic> 0.001), increased [ <jats:italic>P<\/jats:italic><jats:sub><jats:italic>i<\/jats:italic><\/jats:sub> ] to more than 180% of pretreatment values, and decreased [ H<jats:sup>+<\/jats:sup>] by less than 15% (<jats:italic>P<\/jats:italic> 0.05). A significant relative shift in the \u03b1\u2010P and \u03b2\u2010P resonances of ATP (<jats:italic>P<\/jats:italic> 0.01) occurred with inotropic stimulation. [Mg<jats:sup>2+<\/jats:sup>], calculated on the basis of these shifts was found to be 0.78 \u00b1 0.1 m<jats:italic>M<\/jats:italic> in control hearts, and increased to maxima of 1.9 \u00b1 0.2,2.0 \u00b10.2, and 2.9 \u00b1 0.2 m <jats:italic>M<\/jats:italic> during infusion of isoproterenol, IBMX, and ouabain, respectively. Changes in [Mg<jats:sup>2+<\/jats:sup>], correlate with cytosolic [ATP] + [CrP] in all hearts (<jats:italic>r<\/jats:italic> = 0.89, 0.91, and 0.88 in isoproterenol\u2010, IBMX\u2010, and ouabain\u2010treated hearts, respectively). The significantly higher [Mg<jats:sup>2+<\/jats:sup>], with ouabain infusion (<jats:italic>P<\/jats:italic> 0.01) at similar workloads and [ATP] + [CrP] supports the proposal that a ouabain\u2010inhibited Mg<jats:sup>2+<\/jats:sup> pump exists in the plasma membrane. The data support acute changes in [ Mg<jats:sup>2+<\/jats:sup> ]<jats:sub>i<\/jats:sub> during alterations in inotropic state that may be important in modulating metabolic and contractile function. \u00a9 1989 Academic Press, Inc.<\/jats:p>","DOI":"10.1002\/mrm.1910120305","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T10:20:33Z","timestamp":1173090033000},"page":"328-338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Effect of inotropic stimulation on cytosolic mg<sup>2+<\/sup> in isolated rat heart: a <sup>31<\/sup>p magnetic resonance study"],"prefix":"10.1002","volume":"12","author":[{"given":"John P","family":"Headrick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger J.","family":"Willis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1986.sp003005"},{"key":"e_1_2_1_3_2","first-page":"93","volume":"27","author":"Blatter L. A.","year":"1986","journal-title":"Japan. Heart J. Suppl."},{"key":"e_1_2_1_4_2","volume":"254","author":"Godt R. E.","journal-title":"Amer. J. Physiol."},{"key":"e_1_2_1_4_3","first-page":"C591","volume":"23","year":"1988","journal-title":"Cell Physiol."},{"key":"e_1_2_1_5_2","first-page":"265","volume":"15","author":"Gupta R. K.","year":"1983","journal-title":"Physiol. Chem. Phys. Med. NMR"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"14420","DOI":"10.1016\/S0021-9258(18)66886-3","volume":"261","author":"Kushmerick M. J.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_7_2","first-page":"4","volume":"20","author":"Murphy E.","year":"1988","journal-title":"J. Mol. Cell. Cardiol."},{"key":"e_1_2_1_8_2","first-page":"23","volume":"3547","author":"Garfinkel L.","year":"1984","journal-title":"Biochemistry"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014447"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.71.6.645"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00316a036"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01907579"},{"key":"e_1_2_1_13_2","first-page":"257","volume":"18","author":"Ebel H.","year":"1980","journal-title":"J. Clin. Chem. Clin. Biochem."},{"key":"e_1_2_1_14_2","first-page":"60","volume":"4","author":"Garfinkel L.","year":"1985","journal-title":"Magnesium"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35825-8"},{"key":"e_1_2_1_16_2","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S0021-9258(19)35418-3","volume":"263","author":"Vink R.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_17_2","volume":"251","author":"Ford G. F.","journal-title":"Amer. J. Physiol."},{"key":"e_1_2_1_17_3","first-page":"C687","volume":"20","year":"1986","journal-title":"Cell Physiol."},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1974.sp010723"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85622-3"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.13.060184.001253"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(85)80036-5"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(87)80525-4"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/15.6.218"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)80584-1"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1986.tb09854.x"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(86)90132-5"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00402a003"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(82)90205-X"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)81382-5"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(84)90372-9"},{"key":"e_1_2_1_31_2","first-page":"6","volume":"9","author":"Altura B. T.","year":"1987","journal-title":"Magn. Bull."},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1982.tb00942.x"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7361117"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(83)90363-6"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0162-0134(00)80123-3"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.12.060183.000515"},{"key":"e_1_2_1_37_2","volume":"243","author":"Achs M. J.","journal-title":"Amer. J. Physiol."},{"key":"e_1_2_1_37_3","first-page":"R389","volume":"12","year":"1982","journal-title":"Regul. Integrative Comp. Physiol."},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01592a009"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00964a002"},{"key":"e_1_2_1_40_2","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/0006-3002(61)90374-2","volume":"54","author":"Nanninga L. B.","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50400-2"},{"key":"e_1_2_1_42_2","first-page":"241","volume":"1","author":"Altura B. M.","year":"1982","journal-title":"Magnesium"}],"container-title":["Magnetic Resonance in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrm.1910120305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrm.1910120305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T06:43:07Z","timestamp":1697956987000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrm.1910120305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,12]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,12]]}},"alternative-id":["10.1002\/mrm.1910120305"],"URL":"https:\/\/doi.org\/10.1002\/mrm.1910120305","archive":["Portico"],"relation":{},"ISSN":["0740-3194","1522-2594"],"issn-type":[{"value":"0740-3194","type":"print"},{"value":"1522-2594","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,12]]},"assertion":[{"value":"1988-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-11-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}