{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:54:23Z","timestamp":1760853263120},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,5]],"date-time":"2005-12-05T00:00:00Z","timestamp":1133740800000},"content-version":"vor","delay-in-days":4966,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ability of lipid\u2010soluble nitroxides to suppress selectively the peaks of lipid resonances in <jats:sup>31<\/jats:sup>P, <jats:sup>1<\/jats:sup>H, and <jats:sup>13<\/jats:sup>C NMR spectra was investigated in serum as part ofstudies aimed at using these contrast agents for magnetic resonance imaging and magnetic resonance spectroscopy <jats:italic>in vivo.<\/jats:italic> Nitroxides are especially interesting potential contrast agents because they can reversibly be converted in cells to diamagnetic hydroxylamines, with conversion rates that are dependent on the redox potential and the intracellular concentration of oxygen; the characterization of nitroxide\u2010dependent changes in NMR spectra may therefore be a useful means to measureoxygen\u2010dependent redox metabolism <jats:italic>in vivo.<\/jats:italic> The fatty acid analogs, doxyl stearates, suppressed the methyl resonance of choline and the methyl and methylene peaks of lipids in the <jats:sup>1<\/jats:sup>H NMR spectra of serum samples. As a consequence, lactate peaks, which were not readily detected became clearly resolved and could be evaluated quantitatively. The <jats:sup>31<\/jats:sup>P resonance of phosphatidylcholine in the <jats:sup>31<\/jats:sup>P NMR spectrum was suppressed by 5\uf8ffdoxyl stearate and 4\u2010(N,N\uf8ffdimethyl\u2010N\uf8ffhexadecyl)ammonium\u20102,2,6,6\u2010tetramethylpiperidine\u2010I\u2010oxyl, iodide (Cat<jats:sub>16<\/jats:sub>). In the <jats:sup>13<\/jats:sup>C NMR spectrum, the resonances of the methyl groups of choline and the lipids also were broadened significantly by addition of 5\u2010doxyl stearate. Differential suppression of lipid resonances can be employed to facilitate quantitation of lactate.<\/jats:p>","DOI":"10.1002\/mrm.1910250112","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T13:44:28Z","timestamp":1173102268000},"page":"120-127","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Selective suppression of lipid resonances by lipid\u2010soluble nitroxides in NMR spectroscopy"],"prefix":"10.1002","volume":"25","author":[{"given":"Kai","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert W.","family":"Lutz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janna P.","family":"Wehrle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry D.","family":"Glickson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold M.","family":"Swartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,5]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"49","volume-title":"Advances in Magnetic Resonance Imaging","author":"Swartz H. 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