{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:38:08Z","timestamp":1773301088680,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":2994,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The authors have shown direct, real\u2010time, <jats:italic>in vivo<\/jats:italic> measurement of nitric oxide (NO) in mice by using the water soluble metal chelator complex, <jats:italic>N<\/jats:italic>\u2010methyl\u2010D\u2010glucamine dithiocarbamate (MGD), and Fe(II) as monitored by EPR at L\u2010band. The three\u2010line EPR spectrum from the product [(MGD)<jats:sub>2<\/jats:sub>\u2010Fe(II)\u2010NO] was observed noninvasively in lipopolysaccharide (LPS)\u2010treated mice. The spectrum was markedly suppressed by the administration, before LPS injection, of phenyl <jats:italic>N<\/jats:italic>\u2010tert\u2010butyl nitrone (PBN), an inhibitor of the expression of induced nitric oxide synthase (iNOS). When <jats:sup>15<\/jats:sup>N\u2010arginine was administered to LPS\u2010treated mice, a diagnostic EPR spectrum was observed, consisting of both three\u2010 and two\u2010line EPR signals, due to (MGD)<jats:sub>2<\/jats:sub>\u2010Fe(II)\u2010<jats:sup>14<\/jats:sup>NO and (MGD)<jats:sub>2<\/jats:sub>\u2010Fe(II)\u2010<jats:sup>16<\/jats:sup>NO, respectively. The results strongly suggested that the NO detected in these experiments was synthesized by iNOS. <jats:italic>In vivo<\/jats:italic> EPR measurements of [(MGD)<jats:sub>2<\/jats:sub>\u2010Fe(II)\u2010NO] at several regions in the body (from the head to the tail) indicated that the NO was generated mostly in the upper abdomen near the liver. These observations were confirmed by <jats:italic>ex vivo<\/jats:italic> EPR measurements on isolated organs where higher NO levels were detected <jats:italic>in vivo<\/jats:italic> in the liver and kidney. The spectroscopic results, combined with the pharmacokinetic data, support the model that NO detected in LPS\u2010treated mice was produced mainly in the liver, and that it did not reflect NO\u2010adduct complex accumulated in the liver via the blood circulation.<\/jats:p>","DOI":"10.1002\/mrm.1910380410","type":"journal-article","created":{"date-parts":[[2007,3,13]],"date-time":"2007-03-13T07:06:57Z","timestamp":1173769617000},"page":"565-568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Determination and characterization of nitric oxide generation in mice by <i>in vivo<\/i> l\u2010band EPR spectroscopy"],"prefix":"10.1002","volume":"38","author":[{"given":"Hirotada","family":"Fujii","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janusz","family":"Koscielniak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence J.","family":"Berliner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.12.1381691"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470123119.ch8"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/10715769009109680"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(91)90251-6"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90708-S"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49865-7"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(95)00092-7"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.2170"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00422-6"},{"key":"e_1_2_1_11_2","first-page":"65","volume-title":"EPR Imaging and In\u2010Vivo EPR","author":"Berliner L. 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