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A mathematical representation of the spectral\u2010peak shape, including the separation and width\u2010at\u2010half\u2010height in the <jats:sup>31<\/jats:sup>P NMR spectra, as a function of rotor speed, enabled us to explore the extent to which a change in cell volume would be reflected in the spectra if it occurred. We concluded that a fractional volume change in excess of 3% would have been detected by our experiments. Thus, the experiments indicated that the mean cell volume did not change by this amount even at the highest spinning rate of 7 kHz. The mean cell volume and intracellular <jats:sup>31<\/jats:sup>P line\u2010width were independent of the packing density of the cells and of the initial cell volume. The relationship of these conclusions to other non\u2010NMR studies of pressure effects on cells is noted. 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