{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:46:54Z","timestamp":1789984014982,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T00:00:00Z","timestamp":1162771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vitamin C (ascorbate) and glutathione (GSH) are the two most concentrated non\u2010enzymatic antioxidants in the human brain. Double editing with (DEW) MEGA\u2010PRESS at 4T was designed in this study to measure both antioxidants in the same amount of time previously required to measure one. In the occipital lobe of four human subjects, resolved ascorbate (Asc) and GSH resonances were detected repeatedly and simultaneously using DEW MEGA\u2010PRESS. The Asc and GSH concentrations measured using LCModel analysis of DEW MEGA\u2010PRESS spectra were 0.8 \u00b1 0.1 and 1.0 \u00b1 0.1 \u03bcmol\/g (mean \u00b1 SD), with average Cramer\u2010Rao lower bounds (CRLB) of 10% and 7%, respectively. Aside from the effects of <jats:italic>J<\/jats:italic>\u2010modulation at a common echo time (TE), double editing did not compromise sensitivity. To determine the extent to which the oxidized forms of Asc and GSH contribute to DEW MEGA\u2010PRESS spectra in vivo, chemical shifts and coupling constants for dehydroascorbate (DHA) and oxidized glutathione (GSSG) were measured at physiologic pH and temperature. DHA does not contribute to the 3.73 ppm DEW MEGA\u2010PRESS Asc resonance. GSSG contributions to the DEW MEGA\u2010PRESS GSH resonance (3.0 ppm) are negligible under physiologic conditions, and would be evidenced by a distinct GSSG resonance (3.3 ppm) at exceptionally high concentrations. Magn Reson Med, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrm.21086","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T16:18:01Z","timestamp":1162829881000},"page":"1192-1199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Detection of an antioxidant profile in the human brain in vivo via double editing with MEGA\u2010PRESS"],"prefix":"10.1002","volume":"56","author":[{"given":"Melissa","family":"Terpstra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Marjanska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre\u2010Gilles","family":"Henry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Tk\u00e1\u010d","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Gruetter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(97)00347-3"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(99)01543-X"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0502-445"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1074"},{"key":"e_1_2_7_6_2","first-page":"337","volume-title":"7.3 J difference editing. 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