{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:42:50Z","timestamp":1760982170522},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,5,29]],"date-time":"2007-05-29T00:00:00Z","timestamp":1180396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We present a method for MR detection of <jats:sup>17<\/jats:sup>O\u2010labeled phosphate groups. The method employs the <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> relaxivity effect of <jats:sup>17<\/jats:sup>O on <jats:sup>31<\/jats:sup>P nuclei to distinguish between <jats:sup>17<\/jats:sup>O\u2010labeled and unlabeled phosphate groups, and uses spin\u2010echo (SE) acquisitions with RF decoupling at the <jats:sup>17<\/jats:sup>O frequency to generate <jats:sup>31<\/jats:sup>P spectra that show only <jats:sup>17<\/jats:sup>O\u2010labeled phosphate groups. The method provides an alternative to spin\u2010labeling experiments, which are limited to the study of rapidly exchanging phosphate groups by the <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> relaxation rates of phosphorus nuclei. We demonstrate separation of MR signals from labeled and unlabeled phosphate\u2010containing compounds, and characterization of the <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> effect of <jats:sup>17<\/jats:sup>O on phosphate nuclei in <jats:sup>17<\/jats:sup>O\u2010labeled phosphate groups. Previous <jats:sup>17<\/jats:sup>O and <jats:sup>18<\/jats:sup>O phosphate\u2010labeled studies used mass spectrometry or high\u2010resolution MR spectroscopy (MRS) to detect the presence of an isotopic label, which requires ex vivo sample preparation. In our method the detection of <jats:sup>17<\/jats:sup>O\u2010labeled phosphate is manifested as a large change in <jats:sup>31<\/jats:sup>P <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>, and thus allows in vivo detection using simple MR methods. Thus this method may have potential for in vivo studies of bioenergetics and the metabolism of phosphate\u2010containing compounds. Magn Reson Med 57:1168\u20131172, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrm.21226","type":"journal-article","created":{"date-parts":[[2007,5,29]],"date-time":"2007-05-29T18:57:00Z","timestamp":1180465020000},"page":"1168-1172","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of <sup>17<\/sup>O\u2010tagged phosphate by <sup>31<\/sup>P MRS: A method with potential for in vivo studies of phosphorus metabolism"],"prefix":"10.1002","volume":"57","author":[{"given":"Peter E.","family":"Thelwall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,29]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560041127"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.841"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)80916-2"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00439a036"},{"key":"e_1_2_7_6_2","unstructured":"SamuelDWD MeshorerA WassermanI.Preliminary experimental results on the effect of oxygen\u201018 on mice. 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