{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T01:42:07Z","timestamp":1783820527106,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,7,27]],"date-time":"2010-07-27T00:00:00Z","timestamp":1280188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["DFG-SFB 688"],"award-info":[{"award-number":["DFG-SFB 688"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Berufsverband Deutscher Internisten"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study reports the <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> relaxation rates of rhodamine\u2010labeled anionic magnetic nanoparticles determined at 7, 11.7, and 17.6 T both in solution and after cellular internalization. Therefore cells were incubated with rhodamine\u2010labeled anionic magnetic nanoparticles and were prepared at decreasing concentrations. Additionally, rhodamine\u2010labeled anionic magnetic nanoparticles in solution were used for extracellular measurements. <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> were determined at 7, 11.7, and 17.6 T. <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> times were determined with an inversion\u2010recovery snapshot\u2010flash sequence. <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> times were obtained from a multispin\u2010echo sequence. Inductively coupled plasma\u2010mass spectrometry was used to determine the iron content in all samples, and <jats:italic>r<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>r<\/jats:italic><jats:sub>2<\/jats:sub> were subsequently calculated. The results were then compared with cells labeled with AMI\u201025 and VSOP C\u2010200. In solution, the <jats:italic>r<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>r<\/jats:italic><jats:sub>2<\/jats:sub> of rhodamine\u2010labeled anionic magnetic nanoparticles were 4.78\/379 (7 T), 3.28\/389 (11.7 T), and 2.00\/354 (17.6 T). In cells, the <jats:italic>r<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>r<\/jats:italic><jats:sub>2<\/jats:sub> were 0.21\/56 (7 T), 0.19\/37 (11.7 T), and 0.1\/23 (17.6 T). This corresponded to an 11\u2010 to 23\u2010fold decrease in <jats:italic>r<\/jats:italic><jats:sub>1<\/jats:sub> and an 8\u2010 to 15\u2010fold decrease in <jats:italic>r<\/jats:italic><jats:sub>2<\/jats:sub>. A decrease in <jats:italic>r<\/jats:italic><jats:sub>1<\/jats:sub> was observed for AMI\u201025 and VSOP C\u2010200. AMI\u201025 and VSOP exhibited a 2\u2010 to 8\u2010fold decrease in <jats:italic>r<\/jats:italic><jats:sub>2<\/jats:sub>. In conclusion, cellular internalization of iron oxide nanoparticles strongly decreased their <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> potency. Magn Reson Med, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mrm.22557","type":"journal-article","created":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T00:09:40Z","timestamp":1280275780000},"page":"1607-1615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Intracellular and extracellular <i>T<\/i><sub>1<\/sub> and <i>T<\/i><sub>2<\/sub> relaxivities of magneto\u2010optical nanoparticles at experimental high 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