{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:37:01Z","timestamp":1788871021375,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,7,11]],"date-time":"2011-07-11T00:00:00Z","timestamp":1310342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"crossref","award":["2008-201842"],"award-info":[{"award-number":["2008-201842"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100011878","name":"Flemish government","doi-asserted-by":"crossref","award":["SBO-IWT-060838"],"award-info":[{"award-number":["SBO-IWT-060838"]}],"id":[{"id":"10.13039\/501100011878","id-type":"DOI","asserted-by":"crossref"}]},{"name":"K.U.Leuven Center of Excellence \u201cIMIR\u201d"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We aimed to setup a noninvasive and well\u2010controlled methodology for evaluation of the cerebrovascular response in mice (C57BL\/6J; 12 weeks). Therefore we applied a normo\u2010, hypo\u2010, and hyperventilation paradigm combined with arterial spin labeling and monitoring of the expired CO\n                    <jats:sub>2<\/jats:sub>\n                    (expCO\n                    <jats:sub>2<\/jats:sub>\n                    ) (\n                    <jats:italic>n<\/jats:italic>\n                    = 7) or arterial pCO\n                    <jats:sub>2<\/jats:sub>\n                    (apCO\n                    <jats:sub>2<\/jats:sub>\n                    ) (\n                    <jats:italic>n<\/jats:italic>\n                    = 12). Reducing the tidal volume by 25% and the respiratory rate by 20% resulted in hypercapnia (apCO\n                    <jats:sub>2<\/jats:sub>\n                    from 33 \u00b1 6 mmHg to 64 \u00b1 16 mmHg). Increasing the respiratory rate by 25% and the tidal volume by 20% decreased apCO\n                    <jats:sub>2<\/jats:sub>\n                    to 22 \u00b1 5 mmHg. Cerebral blood flow (CBF) was 82 \u00b1 21, 163 \u00b1 41 and 64 \u00b1 18 mL\/100 g\/min during normo, hypo\u2010, and hyperventilation, respectively (midbrain). The correlation of apCO\n                    <jats:sub>2<\/jats:sub>\n                    and CBF levels resulted in a cerebrovascular response of 2.7 \u00b1 0.3, 2.1 \u00b1 0.3, 2.1 \u00b1 0.3, and 3.7 \u00b1 0.5 mL\/100 g\/min\/mmHg for midbrain, cortex, hippocampus and thalamus, respectively. As expCO\n                    <jats:sub>2<\/jats:sub>\n                    levels were correlated with apCO\n                    <jats:sub>2<\/jats:sub>\n                    (\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    = 0.86;\n                    <jats:italic>n<\/jats:italic>\n                    = 4) and CBF (\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    = 0.67) a cerebrovascular response based on simultaneously recorded CBF and expCO\n                    <jats:sub>2<\/jats:sub>\n                    levels could be derived (3.3 \u00b1 0.5, 2.5 \u00b1 0.4, 3.0 \u00b1 0.4, and 4.5 \u00b1 0.6 mL\/100 g\/min\/mmHg; order as above). A cross\u2010over experiment resulted in similar responses. In conclusion, this protocol allows evaluating basal CBF and cerebrovascular response in mice under well\u2010controlled conditions by simply changing ventilator settings and correlating CBF with apCO\n                    <jats:sub>2<\/jats:sub>\n                    and\/or simultaneously obtained expCO\n                    <jats:sub>2<\/jats:sub>\n                    . Magn Reson Med, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/mrm.23060","type":"journal-article","created":{"date-parts":[[2011,7,11]],"date-time":"2011-07-11T14:05:32Z","timestamp":1310393132000},"page":"802-811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Response of mouse brain perfusion to hypo\u2010 and hyperventilation measured by arterial spin labeling"],"prefix":"10.1002","volume":"66","author":[{"given":"W. 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