{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:26:47Z","timestamp":1760984807006},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,5,9]],"date-time":"2013-05-09T00:00:00Z","timestamp":1368057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["DP2 OD004362","R21 NS065297"],"award-info":[{"award-number":["DP2 OD004362","R21 NS065297"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Endogenous labeling of stem\/ progenitor cells via intracerebroventricular injection of micron\u2010sized particles of iron oxide (MPIOs) has become standard methodology for MRI of adult neurogenesis. While this method is well characterized in the na\u00efve rodent brain, it has not been fully investigated in disease models. Here, we describe methodological challenges that can confound data analysis when this technique is applied to a rat model of stroke, the endothelin\u20101 model of focal cortical ischemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We intended to track endogenous neuroblast migration from the subventricular zone to the stroke area using previously described methods for in vivo labeling of endogenous neuroblasts with MPIOs and following migration with high resolution MRI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MPIOs accumulation in stroke regions of endothelin\u20101\u2010treated brains involves two dynamic steps: an initial rapid cell independent mechanism, followed by slower MPIOs accumulation. While the latter may in part be attributable to cell dependent delivery of the particles, the cell independent mechanism complicates the interpretation of the data. Strategies aimed at prelabeling the stem cell niche reduced cell independent MPIOs accumulation, but failed to abolish it.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that MRI\u2010based neural stem\/progenitor cell tracking via direct injection of MPIOs into the lateral and third ventricles, requires significant validation in models of brain disease\/trauma. <jats:bold>Magn Reson Med 71:1568\u20131574, 2014. \u00a9 2013 Wiley Periodicals, Inc<\/jats:bold>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrm.24788","type":"journal-article","created":{"date-parts":[[2013,5,9]],"date-time":"2013-05-09T14:40:25Z","timestamp":1368110425000},"page":"1568-1574","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Accumulation of micron sized iron oxide particles in endothelin\u20101 induced focal cortical ischemia in rats is independent of cell 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