{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T03:39:35Z","timestamp":1768880375489,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,10,4]],"date-time":"2013-10-04T00:00:00Z","timestamp":1380844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01NS070909"],"award-info":[{"award-number":["R01NS070909"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01HD074593"],"award-info":[{"award-number":["R01HD074593"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01HD070996"],"award-info":[{"award-number":["R01HD070996"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>To investigate if frequency\u2010dependent contrasts using oscillating gradient diffusion MRI (dMRI) can detect hypoxia\u2013ischemia (HI) \u2010induced neurodegeneration in the neonatal mouse hippocampus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pulsed\u2010 and oscillating\u2010gradient dMR images (at 50, 100, and 150 H<jats:sc>z<\/jats:sc>) were acquired from postmortem fixed brains of mice exposed to neonatal HI using the Rice\u2010Vanucci model. MRI data were acquired at 1, 4, and 8 days following HI, and compared with histological data from the same mice for in situ histological validation of the MRI findings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The rate of change of apparent diffusion coefficient with gradient frequency (\u0394<jats:sub>f<\/jats:sub>ADC) revealed unique layer\u2010specific contrasts in the neonatal mouse hippocampus. \u0394<jats:sub>f<\/jats:sub>ADC measurements were found to show a significant decrease in response to neonatal HI injury, in the pyramidal (Py) and granule (GrDG) cell layers compared with contralateral regions. The areas of reduced intensity in the \u0394<jats:sub>f<\/jats:sub>ADC maps corresponded to regional neurodegeneration seen with H&amp;E and Fluoro\u2010Jade C stainings, indicating that alterations in \u0394<jats:sub>f<\/jats:sub>ADC contrasts are sensitive to early microstructural changes due to HI\u2010induced neurodegeneration in the studied regions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings show that the frequency\u2010dependence of ADC measurements with oscillating\u2010gradient dMRI can provide a sensitive contrast to detect HI\u2010induced neurodegeneration in neuronal layers of the neonatal mouse hippocampus. Magn Reson Med 72:829\u2013840, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrm.24956","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T02:20:15Z","timestamp":1381544415000},"page":"829-840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Imaging neurodegeneration in the mouse hippocampus after neonatal hypoxia\u2013ischemia using oscillating gradient diffusion MRI"],"prefix":"10.1002","volume":"72","author":[{"given":"Manisha","family":"Aggarwal","sequence":"first","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science Johns Hopkins University School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Burnsed","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Johns Hopkins University School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee J.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Johns Hopkins University School of Medicine Baltimore Maryland USA"},{"name":"Division of Neuropathology Johns Hopkins University School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frances J.","family":"Northington","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Johns Hopkins University School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science Johns Hopkins University School of Medicine Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,4]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041996"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.47.1.32"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19990115)55:2<158::AID-JNR3>3.0.CO;2-1"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2000.0371"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.29.3.695"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-21-07994.2000"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(01)01788-8"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.03.062"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.20439"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600385"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3883-04.2004"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.58.12.1726"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.107.506352"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000014608.78503.DB"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000137768.25203.df"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21517"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.107.509927"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2010.168"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22869"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1531"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmre.2000.2203"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4363(81)90182-0"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21793"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.20732"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2006.06.023"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0730-725X(03)00155-3"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10385"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2010.10.003"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0832"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24325"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22981"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410090206"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199602000-00003"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22426"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910350319"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2010.07.043"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004728-199801000-00027"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.4.092101.125733"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2004.11.054"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(81)90016-X"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(98)00111-4"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22407"},{"key":"e_1_2_5_44_1","unstructured":"McHughDJ HubbardPL ZhaoS ParkerGJ NaishJH.Distinguishing restricted diffusion and flow using pulsed and oscillating diffusion gradients at 1.5T. In Proceedings of the 20th Annual Meeting of the ISMRM Melbourne Australia 2012. p.4109."},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24632"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21977"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.20488"}],"container-title":["Magnetic Resonance in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrm.24956","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmrm.24956","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mrm.24956","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T18:29:19Z","timestamp":1696357759000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mrm.24956"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,4]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1002\/mrm.24956"],"URL":"https:\/\/doi.org\/10.1002\/mrm.24956","archive":["Portico"],"relation":{},"ISSN":["0740-3194","1522-2594"],"issn-type":[{"value":"0740-3194","type":"print"},{"value":"1522-2594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,4]]},"assertion":[{"value":"2013-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}