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Spatial maps of pH were quantified in vitro, with a focus on method\u2010based errors, and applied in vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In vitro and in vivo\n                      <jats:sup>13<\/jats:sup>\n                      C mapping were performed for various flip angles for bicarbonate (BiC) and CO\n                      <jats:sub>2<\/jats:sub>\n                      with spectral\u2010spatial excitation and spiral readout in healthy Lewis rats in five slices. Acute subcutaneous sterile inflammation was induced with Concanavalin A in the right leg of Buffalo rats. pH and proton images were measured 2 h after induction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After optimizing the signal to noise ratio of the hyperpolarized\n                      <jats:sup>13<\/jats:sup>\n                      C\u2010bicarbonate, error estimation of the spectral\u2010spatial excited spectrum reveals that the method covers the biologically relevant pH range of 6 to 8 with low pH error (&lt; 0.2). Quantification of pH maps shows negligible impact of the residual bicarbonate signal. pH maps reflect the induction of acute metabolic alkalosis. Inflamed, infected regions exhibit lower pH.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Hyperpolarized\n                      <jats:sup>13<\/jats:sup>\n                      C\u2010bicarbonate pH mapping was shown to be sensitive in the biologically relevant pH range. The mapping of pH was applied to healthy in vivo organs and interpreted within inflammation and acute metabolic alkalosis models. Magn Reson Med 73:2274\u20132282, 2015. \u00a9 2014 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mrm.25357","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T15:40:31Z","timestamp":1405438831000},"page":"2274-2282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Quantified p\n                    <scp>H<\/scp>\n                    imaging with hyperpolarized\n                    <sup>13<\/sup>\n                    <scp>C<\/scp>\n                    \u2010bicarbonate"],"prefix":"10.1002","volume":"73","author":[{"given":"David Johannes","family":"Scholz","sequence":"first","affiliation":[{"name":"Technische Universit\u00e4t M\u00fcnchen Institute of Medical Engineering Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin 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