{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:34:58Z","timestamp":1779244498838,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T00:00:00Z","timestamp":1411948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01-AG034953"],"award-info":[{"award-number":["R01-AG034953"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01-EB014861"],"award-info":[{"award-number":["R01-EB014861"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P30-NS052519"],"award-info":[{"award-number":["P30-NS052519"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100001453","name":"American Brain Tumor Association","doi-asserted-by":"crossref","award":["2013 Discovery Grant"],"award-info":[{"award-number":["2013 Discovery Grant"]}],"id":[{"id":"10.13039\/100001453","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Carbon\u201013 (<jats:sup>13<\/jats:sup>C) magnetic resonance spectroscopy (MRS) has an intrinsically low NMR sensitivity that often leads to large acquisition volumes or long scan times. While the use of higher magnetic fields can overcome the sensitivity limitations, high radiofrequency (RF) power deposition associated with proton\u2010decoupling limits the achievable gain. Two\u2010dimensional (2D) heteronuclear single quantum coherence (HSQC) MRS is a method that uses the high chemical specificity of <jats:sup>13<\/jats:sup>C MRS while retaining the high sensitivity of <jats:sup>1<\/jats:sup>H detection. Due to the 2D nature of the method, proton\u2010decoupled <jats:sup>13<\/jats:sup>C MR spectra can be obtained without the use of high\u2010powered decoupling pulses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A novel three\u2010dimensional (3D) localized 2D HSQC method based on 3D STEAM localization is presented and implemented at 7T. The low RF power deposition of the method allows TR variation along the indirect dimension which, in combination with controlled aliasing, leads to an acceleration of 11.8 relative to a standard 2D NMR acquisition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Artifact\u2010free, high\u2010quality and high\u2010sensitivity 2D HSQC spectra were obtained for all subjects in 19 min from a small (9 mL) volume placed in the leg adipose tissue. Complete proton decoupling was achieved along the indirect <jats:sup>13<\/jats:sup>C dimension despite the absence of broadband proton\u2010decoupling pulses. The high chemical specificity along the indirect <jats:sup>13<\/jats:sup>C dimension allowed the detection of 19 unique resonances from which the lipids could be characterized in terms of saturation and omega\u20106\/omega\u20103 fatty acid ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It has been demonstrated that high\u2010quality 2D HSQC NMR spectra can be acquired from human adipose tissue at 7T. The HSQC method is methodologically simple and robust and is flexible regarding trade\u2010offs between temporal and spectral resolution. 2D HSQC has a strong potential to become a default method in natural\u2010abundance or <jats:sup>13<\/jats:sup>C\u2010enriched studies of human metabolism in vivo. Magn Reson Med 74:903\u2013914, 2015. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrm.25470","type":"journal-article","created":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T13:23:05Z","timestamp":1411996985000},"page":"903-914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["High\u2010sensitivity, broadband\u2010decoupled <sup>13<\/sup>C MR spectroscopy in humans at 7T using two\u2010dimensional heteronuclear single\u2010quantum coherence"],"prefix":"10.1002","volume":"74","author":[{"given":"Robin A.","family":"de Graaf","sequence":"first","affiliation":[{"name":"Department of Diagnostic Radiology Magnetic Resonance Research Center, Yale University School of Medicine New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk M.","family":"De Feyter","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology Magnetic 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