{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:10Z","timestamp":1786978390516,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["34038"],"award-info":[{"award-number":["34038"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["355795"],"award-info":[{"award-number":["355795"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Magnetic Resonance in Med"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To develop a facile method for labeling and imaging decellularized extracellular matrix (dECM) scaffolds intended for regenerating 3D tissues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A small molecule manganese porphyrin, MnPNH\n                      <jats:sub>2<\/jats:sub>\n                      , was synthesized and used to label dECM scaffolds made from porcine bladder and trachea and murine whole lungs. The labeling protocol was optimized on bladder dECM, and imaging on a 3T clinical scanner was performed to assess reductions in\n                      <jats:italic>T<\/jats:italic>\n                      <jats:sub>1<\/jats:sub>\n                      and\n                      <jats:italic>T<\/jats:italic>\n                      <jats:sub>2<\/jats:sub>\n                      relaxation times. In vivo MRI was performed on dECM injected in the rat dorsum to verify sensitivity of detection. Toxicity assays for cell viability, metabolism, and proliferation were performed on human umbilical vein endothelial cells. The incorporation of MnPNH\n                      <jats:sub>2<\/jats:sub>\n                      and its long\u2010term retention in dECM were assessed on transmission electron microscopy and ultraviolet absorbance of eluted MnPNH\n                      <jats:sub>2<\/jats:sub>\n                      over time.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All tissues, including thick whole 3D organs, were uniformly labeled and demonstrated high signal\u2010to\u2010noise on MRI. A nearly 10\u2010fold reduction in\n                      <jats:italic>T<\/jats:italic>\n                      <jats:sub>1<\/jats:sub>\n                      was consistently obtained at a labeling dose of 0.4 mM, and even 0.2 mM provided sufficient contrast in vivo and ex vivo. No toxicity was observed up to 0.4 mM, the maximum tested. Binding studies suggested nonspecific association, and retention studies in the labeled whole decellularized lungs revealed less than 20% MnPNH\n                      <jats:sub>2<\/jats:sub>\n                      loss over 30 days, the majority occurring in the first 3 days after labeling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The proposed labeling method is the first report for visualizing dECM on MRI and has the potential for long\u2010term monitoring and optimization of dECM\u2010based organ tissue engineering.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mrm.28072","type":"journal-article","created":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T01:52:14Z","timestamp":1573782734000},"page":"2138-2149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering"],"prefix":"10.1002","volume":"83","author":[{"given":"Daniel Andrzej","family":"Szulc","sequence":"first","affiliation":[{"name":"Institute of Biomaterials &amp; Biomedical Engineering University of Toronto Canada"},{"name":"Ted Rogers Centre for Heart Research Translational Biology &amp; Engineering Program Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadali","family":"Ahmadipour","sequence":"additional","affiliation":[{"name":"Institute of Biomaterials &amp; Biomedical Engineering University of Toronto Canada"},{"name":"Latner Thoracic Surgery Laboratories Toronto General Hospital Research Institute University Health Network Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio Gava","family":"Aoki","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Laboratories Toronto General Hospital Research Institute University Health Network Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas K.","family":"Waddell","sequence":"additional","affiliation":[{"name":"Institute of Biomaterials &amp; Biomedical Engineering University of Toronto Canada"},{"name":"Latner Thoracic Surgery Laboratories Toronto General Hospital Research Institute University Health Network Toronto Canada"},{"name":"Department of Mechanical and Industrial Engineering University of Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Golnaz","family":"Karoubi","sequence":"additional","affiliation":[{"name":"Latner Thoracic Surgery Laboratories Toronto General Hospital Research Institute University Health Network Toronto Canada"},{"name":"Department of Mechanical and Industrial Engineering University of Toronto Canada"},{"name":"Ontario Institute for Regenerative Medicine Toronto Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0733-9512","authenticated-orcid":false,"given":"Hai\u2010Ling Margaret","family":"Cheng","sequence":"additional","affiliation":[{"name":"Institute of Biomaterials &amp; Biomedical Engineering University of Toronto Canada"},{"name":"Ted Rogers Centre for Heart Research Translational Biology &amp; Engineering Program Toronto Canada"},{"name":"Ontario Institute for Regenerative Medicine Toronto Canada"},{"name":"Heart &amp; Stroke\/Richard Lewar Centre of Excellence for Cardiovascular Research Toronto Canada"},{"name":"The Edward S. 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