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In vivo studies in a murine adenocarcinoma model (n = 4 mice for each contrast agent) upon i.v. injection provided CEST\u2010derived perfusion tumor properties that were compared with those obtained with a gadolinium\u2010based blood\u2010pool agent (Gd\u2010AAZTA\u2010Madec).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro measurements showed a marked CEST contrast dependency to pH, with higher CEST contrast at lower pH values for both molecules. The measured prototropic exchange rates confirmed a base\u2010catalyzed exchange rate that was faster for dextran 70 in comparison to voluven. Both molecules showed a similar CEST contrast increase (\u0394ST% &gt; 3%) in the tumor tissue up to 30 min postinjection, with heterogeneous accumulation. In tumors receiving both CEST and T<jats:sub>1<\/jats:sub>\u2010weighted agents, a voxel\u2010by\u2010voxel analysis indicated moderate spatial correlation of perfusion properties between voluven\/dextran 70 and Gd\u2010AAZTA\u2010Madec, suggesting different distribution patterns according to their molecular size.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The obtained results showed that both voluven and dextran 70 can be exploited as MRI\u2010CEST contrast agents for evaluating tumor enhancement properties. Their increased accumulation in tumors and prolonged contrast enhancement promote their use as blood\u2010pool MRI\u2010CEST agents to examine tumor vascularization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrm.28778","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T11:25:24Z","timestamp":1616671524000},"page":"995-1007","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Investigating plasma volume expanders as novel macromolecular MRI\u2010CEST contrast agents for tumor contrast\u2010enhanced imaging"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1667-4854","authenticated-orcid":false,"given":"Lorena","family":"Consolino","sequence":"first","affiliation":[{"name":"Molecular Imaging Center Department of Molecular Biotechnology and Health Sciences University of Turin Turin 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