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The method was validated by correlation and Bland\u2010Altman analysis on a ferritin\u2010containing phantom. Mineral\u2010iron maps were obtained from postmortem tissue of patients with neurological diseases characterized by brain iron accumulation, that is, Alzheimer disease, Huntington disease, and aceruloplasminemia, and validated with histology. Transverse relaxation rate and magnetic susceptibility values were used for comparison.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In postmortem tissue, the mineral\u2010iron contrast colocalizes with histological iron staining in all the cases. Iron concentrations obtained via the off\u2010resonance saturation method are in agreement with literature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Off\u2010resonance saturation is an effective way to detect iron in gray matter structures and partially mitigate for the presence of myelin. If a reference region with little iron is available in the tissue, the method can produce quantitative iron maps. This method is applicable in the study of diseases characterized by brain iron accumulation and can complement existing iron\u2010sensitive parametric methods.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mrm.29041","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T19:35:17Z","timestamp":1634758517000},"page":"1276-1288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Off\u2010resonance saturation as an MRI method to quantify mineral\u2010 iron in the post\u2010mortem brain"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3156-4696","authenticated-orcid":false,"given":"Lucia","family":"Bossoni","sequence":"first","affiliation":[{"name":"C. J. 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