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In situ scans were conducted within 32\u2009h after death (SD 18\u2009h), and ex situ scans after brain extraction and 3\u2009months of formalin fixation. The imaging protocol included MP2RAGE, DTI, and multi\u2010contrast spin\u2010echo and multi\u2010echo gradient\u2010echo sequences. Volumetry, fractional anisotropy, mean diffusivity, <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub>, <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>, and  have been assessed for specific brain regions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When comparing ex situ to in situ values, the following results were obtained. Deep gray matter as well as the thalamus and the hippocampus showed a reduced volume. Fractional anisotropy was reduced in the cortex and the whole brain. Mean diffusivity was decreased in white matter and deep gray matter. <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> and <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> were reduced in all investigated structures, whereas  was increased in the cortex.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results of this study show that the volumes and MRI parameters of several brain regions are potentially affected by tissue extraction and subsequent formalin fixation, suggesting that methodological alterations are present in ex situ MRI. 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