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After collecting T\n                      <jats:sub>2<\/jats:sub>\n                      \u2010weighted scans, slab dynamic\n                      <jats:sup>13<\/jats:sup>\n                      C MRS data were acquired. Time\u2010course curves of [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]pyruvate, [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]lactate and [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]lactate\/total carbon ratio were calculated. Mean full\u2010width\u2010half\u2010maximum (FWHM) and area\u2010under\u2010curve (AUC) values were compared across injection times. A simplified one\u2010compartment model of pyruvate metabolism was fit using the conversion rate constant (\n                      <jats:italic>k<\/jats:italic>\n                      <jats:sub>PL<\/jats:sub>\n                      ) and effective lactate decay rate (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sub>\n                        1\n                        <jats:italic>eff<\/jats:italic>\n                      <\/jats:sub>\n                      ). Dynamic EPSI images, acquired using an injection time of 15\u2009s and 60\u2009s for comparison.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean FWHM values of the time\u2010course curves of [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]pyruvate and [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]lactate showed a significant increase (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01) with increasing injection times, while no statistical significance was found across the AUC values. The time\u2010course curves of lactate\/total carbon ratio showed elongated plateaus with increased injection times. Kinetic modeling showed good agreement between fitted and acquired lactate data, with AUC of normalized lactate profile remaining constant across infusion times. Dynamic EPSI images acquired with a longer infusion time (60\u2009s) showed the ability to monitor brain metabolism as it approached pseudo\u2010steady state.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Increased delivery times of hyperpolarized [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]pyruvate dissolved in D\n                      <jats:sub>2<\/jats:sub>\n                      O approaches pseudo\u2010steady state metabolism in vivo and allowing for the potential to cater new acquisition and reconstruction approaches for enhanced imaging.\n                    <\/jats:p>\n                  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