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In an immunodeficient rat model, hPSC\u2010CMs injected into the left ventricular myocardium were tracked on cardiac MRI at 3 Tesla over 8\u2009weeks in both healthy and infarcted hearts. Longitudinal MRI was accompanied by MnCl\n                      <jats:sub>2<\/jats:sub>\n                      supplementation for on\u2010demand recall of bright signal from ferritin\u2010overexpressing hPSC\u2010CMs. MRI findings were corroborated against histological validation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Ferritin\u2010overexpressing hPSC\u2010CMs displayed normal morphological and functional phenotypes and created viable grafts in vivo. On\u2010demand recall of bright contrast on MRI was achieved, regardless of interval post\u2010cell transplantation, via MnCl\n                      <jats:sub>2<\/jats:sub>\n                      administration to precisely map surviving hPSC\u2010CMs in both healthy and infarcted hearts. The spatial distribution of hPSC\u2010CMs on MRI was confirmed throughout the heart by histology. Echocardiography confirmed MnCl\n                      <jats:sub>2<\/jats:sub>\n                      had no impact on cardiac function, although in vitro tests revealed transiently dampened calcium handling and contractility, an effect from which cells fully recovered after removing MnCl\n                      <jats:sub>2<\/jats:sub>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Bright ferritin MRI allows longitudinal, non\u2010invasive, and on\u2010demand imaging of the distribution of viable transplanted hPSC\u2010CMs in the healthy and infarcted rat heart.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mrm.70316","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:02:35Z","timestamp":1772964155000},"page":"323-338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bright Ferritin for Non\u2010Invasive\n                    <scp>MRI<\/scp>\n                    Monitoring of the Fate of Transplanted\n                    <scp>hPSC<\/scp>\n                    \u2010Cardiomyocytes in the Infarcted Rat Heart"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6282-1357","authenticated-orcid":false,"given":"Keyu","family":"Zhuang","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering, University of Toronto  Toronto Ontario Canada"},{"name":"Translational Biology &amp; Engineering Program, Ted Rogers Centre for Heart Research  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8145-4682","authenticated-orcid":false,"given":"Faisal J.","family":"Alibhai","sequence":"additional","affiliation":[{"name":"McEwen Stem Cell Institute, University Health Network  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2482-3354","authenticated-orcid":false,"given":"Beiping","family":"Qiang","sequence":"additional","affiliation":[{"name":"McEwen Stem Cell Institute, University Health Network  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocco","family":"Romagnuolo","sequence":"additional","affiliation":[{"name":"McEwen Stem Cell Institute, University Health Network  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6057-0935","authenticated-orcid":false,"given":"Kyle D. 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