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Using a previously developed bootstrap method and standard cross\u2010spectral analysis, we performed statistical tests to assess the effect of hand position on: (1) the frequency of the EMG; and (2) the phase between the EMGs recorded from antagonistic muscle pairs. Frequency as well as phases changed significantly with different positions of the hands but not during the recordings when the position was left unchanged. Besides confirmation that frequency and phase are stationary and reliable parameters during short\u2010term recordings under controlled laboratory conditions, these results are of particular interest for ambulatory long\u2010term tremor measurements. A higher variability of the estimated parameters reported in long\u2010term recordings may perhaps reflect a patient's mobility only. Our study shows that long\u2010term recording systems should have the means to monitor the patient's movements to provide reliable results. \u00a9 2001 John Wiley &amp; Sons, Inc. 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