{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:04:35Z","timestamp":1793037875011,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,7,22]],"date-time":"2004-07-22T00:00:00Z","timestamp":1090454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>When skeletal muscle is stretched or injured, satellite cells are activated to proliferate, and this process can be mediated by release of the active form of hepatocyte growth factor (HGF) from the extracellular matrix. The objective of these experiments was to determine whether the mechanism of release includes proteolytic activation of pro\u2010HGF. Extracellular HGF in uninjured adult rat extensor digitorum longus muscle was extracted by treatment with 1 M NaCl or heparinases I and III in the presence of a cocktail of serine protease inhibitors. Active HGF heterodimer was the predominant form present, but both pro\u2010HGF and active HGF were extracted when muscle was incubated with Triton X\u2010100 or crush\u2010injured. Incubation of exogenous pro\u2010HGF with uninjured or crush\u2010injured skeletal muscle resulted in cleavage to the active form, indicating that endogenous extracellular proteases are present and capable of rapidly converting pro\u2010HGF to active HGF. Finally, treatment with sodium nitroprusside, a nitric oxide (NO) donor, resulted in liberation of active HGF. These experiments indicate that the active form of HGF is present in the extracellular compartment of uninjured skeletal muscle; therefore, the mechanism of HGF release in response to stretch and NO does not require proteolytic activation of pro\u2010HGF. Muscle Nerve, 2004<\/jats:p>","DOI":"10.1002\/mus.20114","type":"journal-article","created":{"date-parts":[[2004,7,22]],"date-time":"2004-07-22T23:01:14Z","timestamp":1090537274000},"page":"654-658","source":"Crossref","is-referenced-by-count":81,"title":["Active hepatocyte growth factor is present in skeletal muscle extracellular matrix"],"prefix":"10.1002","volume":"30","author":[{"given":"Ryuichi","family":"Tatsumi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald E.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,22]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041650211"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.5.1859"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1089-8603(02)00011-3"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.8.2997"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.44.27558"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007802200"},{"key":"e_1_2_7_8_2","first-page":"949","article-title":"Activation of hepatocyte growth factor by the plasminogen activators uPA and tPA","volume":"143","author":"Mars WM","year":"1993","journal-title":"Am J Pathol"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82167-6"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37062-X"},{"key":"e_1_2_7_11_2","doi-asserted-by":"crossref","first-page":"20114","DOI":"10.1016\/S0021-9258(19)88673-8","article-title":"Activation of hepatocyte growth factor by proteolytic conversion of single chain form to a heterodimer","volume":"267","author":"Naka D","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.17.6489"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.2.603"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209778200"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.6.1783"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(93)90015-5"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(200002)23:2<239::AID-MUS15>3.0.CO;2-U"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6370"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.tb20463.x"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8803"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-01-0062"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2001.5252"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmus.20114","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.20114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:01:55Z","timestamp":1697436115000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.20114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,22]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["10.1002\/mus.20114"],"URL":"https:\/\/doi.org\/10.1002\/mus.20114","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,22]]}}}