{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:21:08Z","timestamp":1770704468798,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Differences in neuromuscular transmission and neuromuscular junction morphology exist across muscle fiber types. We hypothesized that these fiber\u2010type differences are reflected in the size of the cycling synaptic vesicle pool. Synaptic vesicle cycling at type\u2010identified rat diaphragm neuromuscular junctions was examined by fluorescently labeling presynaptic vesicles with FM4\u201064. We found that FM4\u201064 fluorescence uptake was higher at presynaptic terminals of type I\/IIa fibers than type IIx\/IIb fibers. However, no fiber\u2010type differences in the rate of FM4\u201064 destaining were found with repetitive nerve stimulation. Synaptic vesicle density at active zones was examined by transmission electron microscopy. In accordance with FM4\u201064 uptake, synaptic vesicle density was greater at type I\/IIa than IIx\/IIb fibers. These results demonstrate differences in synaptic vesicle cycling across diaphragm muscle fiber types, which may underlie previously observed differences in neuromuscular transmission across diaphragm muscle fiber types. In the diaphragm, motor units comprising type I and type IIa fibers are most frequently recruited with a duty cycle of \u223c40%. Motor units comprising IIx\/IIb fibers are infrequently recruited and only for short durations. The capacity for synaptic vesicle release and cycling at different muscle fiber types matches the functional requirements of these motor units. If the demand for recruitment of motor units comprising IIx\/IIb fibers increases, for example, with mechanical loading, there is an increased risk for neuromuscular transmission failure that my relate to the capacity for synaptic vesicle release and cycling. Muscle fiber type\u2013specific adaptations should be considered when examining neuromuscular disorders. Muscle Nerve, 2004<\/jats:p>","DOI":"10.1002\/mus.20173","type":"journal-article","created":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T23:00:43Z","timestamp":1097535643000},"page":"774-783","source":"Crossref","is-referenced-by-count":38,"title":["Synaptic vesicle cycling at type\u2010identified diaphragm neuromuscular junctions"],"prefix":"10.1002","volume":"30","author":[{"given":"Carlos B.","family":"Mantilla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharine L.","family":"Rowley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Fahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Zhi","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary C.","family":"Sieck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(97)01083-7"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-02-00363.1992"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019472"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.60.1.347"},{"key":"e_1_2_7_6_2","series-title":"Handbook of physiology. 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