{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T15:48:33Z","timestamp":1770479313358,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,3,25]],"date-time":"2005-03-25T00:00:00Z","timestamp":1111708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Anti\u2010GQ1b antibodies are present in the Miller Fisher syndrome (MFS), a monophasic neuropathy characterized by ataxia, areflexia, ophthalmoplegia, and sometimes cranial muscle weakness. We have previously shown, at the mouse neuromuscular junction (NMJ) ex vivo, that anti\u2010GQ1b antibodies, through complement classic pathway activation, block synaptic transmission in a way that resembles the effect of the pore\u2010forming \u03b1\u2010latrotoxin (\u03b1LTx). In order to clarify the mechanism of these \u03b1LTx\u2010like effects, including possible involvement of the alternative and mannose\u2010binding protein complement pathways, we studied the effects of concanavalin A (ConA), a lectin known to block the action of \u03b1LTx, immunoglobulins, and early complement components. With electrophysiological, immunohistological, and bioassay experiments, we showed that the \u03b1LTx\u2010like effects of anti\u2010GQ1b antibody and complement were inhibited by pre\u2010 and coincubation with ConA. However, ConA was not able to inhibit evolution of \u03b1LTx\u2010like effects when coincubated upon addition of complement at NMJs that had already bound anti\u2010GQ1b antibody. Our data suggest that the mannose\u2010binding protein pathway is not involved in the \u03b1LTx\u2010like effect and that the inhibiting effect of ConA principally arises through interference with presynaptic binding of anti\u2010GQ1b antibody. In control experiments, ConA prevented the neuroexocytotic effects of \u03b1LTx, indicating that \u03b1LTx receptors were inhibited under these conditions. We conclude that, although the physiological effects at the NMJ of anti\u2010GQ1b antibody and \u03b1LTx are very similar, the activity of anti\u2010GQ1b antibody is not mediated through activation of \u03b1LTx receptors, but rather is caused by direct presynaptic membrane damage through classic complement pathway activation. Muscle Nerve, 2005<\/jats:p>","DOI":"10.1002\/mus.20327","type":"journal-article","created":{"date-parts":[[2005,3,25]],"date-time":"2005-03-25T17:46:35Z","timestamp":1111772795000},"page":"751-760","source":"Crossref","is-referenced-by-count":9,"title":["Concanavalin a inhibits pathophysiological effects of anti\u2010ganglioside GQ1b antibodies at the mouse neuromuscular synapse"],"prefix":"10.1002","volume":"31","author":[{"given":"Roland W. M.","family":"Bullens","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan K.","family":"Halstead","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham M.","family":"O'Hanlon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Veitch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C.","family":"Molenaar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugh J.","family":"Willison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaap J.","family":"Plomp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00688094"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.71062421.x"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1998.00053.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4598(200007)23:7<1035::AID-MUS5>3.0.CO;2-R"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-16-06876.2002"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.43.10.1911"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20020362"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.10.3844"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39364-X"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6837"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(78)80464-5"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh231"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10060"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.13.3250"},{"key":"e_1_2_7_16_2","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.4049\/jimmunol.118.5.1622","article-title":"Effect of concanavalin A on the classical complement pathway","volume":"118","author":"Langone JJ","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400080102"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.34.21504"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1982.tb06633.x"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02918256"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020284302718"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/124.5.893"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1531-8249(199902)45:2<189::AID-ANA9>3.0.CO;2-T"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(78)90755-2"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/42408"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.24.1.933"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2000.00282.x"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.109.6.1402"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1967.tb09571.x"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.56.11.1178"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.56.2.204"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90121-3"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmus.20327","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.20327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T19:50:43Z","timestamp":1697485843000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.20327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3,25]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,6]]}},"alternative-id":["10.1002\/mus.20327"],"URL":"https:\/\/doi.org\/10.1002\/mus.20327","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3,25]]}}}