{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:29:38Z","timestamp":1761647378690},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,3]],"date-time":"2005-11-03T00:00:00Z","timestamp":1130976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The expression of ganglioside\u2010mimicking structures of <jats:italic>Campylobacter jejuni<\/jats:italic> lipooligosaccharides (LOS) is considered essential for the induction of antiganglioside antibodies that lead to Guillain\u2013Barr\u00e9 syndrome (GBS). The <jats:italic>galE<\/jats:italic> gene in <jats:italic>C. jejuni<\/jats:italic> is involved in the biosynthesis of the LOS outer\u2010core oligosaccharide structures. We have demonstrated that the <jats:italic>C. jejuni<\/jats:italic> HB9313 (HS:19) parental strain expresses a LOS structure containing GM1\u2010like epitopes, and the <jats:italic>C. jejuni<\/jats:italic> knockout mutant of the <jats:italic>galE<\/jats:italic> gene expresses a truncated LOS structure without GM1\u2010like epitopes. To clarify whether the ganglioside\u2010like structures in <jats:italic>Campylobacteri<\/jats:italic> LOS are crucial for induction of antiganglioside antibody responses and neuropathy, we performed immunization experiments in guinea pig models using the parental strain HB9313 and its <jats:italic>galE<\/jats:italic> mutant derivative. The anti\u2010GM1 IgG antibody responses in immunized animals were measured by enzyme\u2010linked immunosorbent assay. Sciatic nerve specimens were evaluated pathologically. High levels of the anti\u2010GM1 IgG antibody were induced in guinea pigs immunized with HB9313, but not in those immunized with the <jats:italic>galE<\/jats:italic> mutant. The mean percentage of abnormality of sciatic\u2010nerve teased fibers from animals sensitized with <jats:italic>C. jejuni<\/jats:italic> HB9313 was significantly higher than from animals immunized with the <jats:italic>galE<\/jats:italic> mutant. Furthermore, significant changes were found in semithin sections of the sciatic nerve from animals inoculated with <jats:italic>C. jejuni<\/jats:italic> HB9313. The major pathological finding was axonal degeneration; no significant morphological findings, except for occasional demyelination, were observed in animals immunized with the <jats:italic>galE<\/jats:italic> mutant. These results indicate that ganglioside\u2010mimicry structures in <jats:italic>C. jejuni<\/jats:italic> LOS are necessary for induction of antiganglioside antibody response and neuropathy. Muscle Nerve 2006<\/jats:p>","DOI":"10.1002\/mus.20457","type":"journal-article","created":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T14:48:03Z","timestamp":1132152483000},"page":"225-231","source":"Crossref","is-referenced-by-count":15,"title":["Carbohydrate mimicry of <i>Campylobacter jejuni<\/i> lipooligosaccharide is critical for the induction of anti\u2010GM1 antibody and neuropathy"],"prefix":"10.1002","volume":"33","author":[{"given":"Xiao Mei","family":"Shu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang Cheng","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,3]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2462-2469.2001"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1128\/iai.62.5.2122-2125.1994","article-title":"Lipopolysaccharides from Campylobacter jejuni associated with Guillain\u2013Barr\u00e9 syndrome patients mimic human gangliosides in structure","volume":"2","author":"Aspinall GO","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00167a032"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0404.2001.103005267.x"},{"key":"e_1_2_7_6_2","first-page":"536","volume-title":"Peripheral neuropathy","author":"Dyck PJ","year":"1993"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.5.2594-2601.2000"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200415707"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6656-6662.2000"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.787-793.2002"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(99)00195-2"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410390404"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01780.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(87)90112-3"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/513781"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/513778"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.451555"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.6078.9"},{"key":"e_1_2_7_18_3","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296548"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awf272"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402391101"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmus.20457","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.20457","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T20:12:12Z","timestamp":1696795932000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.20457"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,3]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1002\/mus.20457"],"URL":"https:\/\/doi.org\/10.1002\/mus.20457","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11,3]]}}}