{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:11:59Z","timestamp":1791223919283,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T00:00:00Z","timestamp":1248220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myotonic dystrophy type 1 (DM1) is a neuromuscular disorder caused by an unstable (CTG \u00b7 CAG)n segment in the 3\u2032 untranslated region of the <jats:italic>myotonic dystrophy protein kinase<\/jats:italic> (<jats:italic>DMPK<\/jats:italic>) gene. It is commonly accepted that <jats:italic>DMPK<\/jats:italic> mRNA\u2010based toxicity is the main contributor to DM1 manifestations; however, not much is known about the significance of the DMPK protein. To appreciate its normal and possible pathobiological role, we analyzed the patterns of DMPK splice isoform expression in mouse tissues. Long membrane\u2013anchored DMPK dominated in heart, diaphragm, and skeletal muscle, whereas short cytosolic isoforms were highly expressed in bladder and stomach. Both isoform types were present in diverse brain regions. DMPK protein was also detectable in cultured myoblasts, myotubes, cortical astrocytes, and related cell lines of neural or muscle origin, but not in hippocampal neurons. This work identifies DMPK as a kinase with pronounced expression in diverse muscle and neural tissues that are affected in DM1. Muscle Nerve, 2009<\/jats:p>","DOI":"10.1002\/mus.21352","type":"journal-article","created":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T18:22:24Z","timestamp":1248286944000},"page":"545-555","source":"Crossref","is-referenced-by-count":22,"title":["DMPK protein isoforms are differentially expressed in myogenic and neural cell lineages"],"prefix":"10.1002","volume":"40","author":[{"given":"Ralph J. A.","family":"Oude Ophuis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan A. M.","family":"Mulders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 E. M. A.","family":"van Herpen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinske","family":"van de Vorstenbosch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9","family":"Wieringa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derick G.","family":"Wansink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,22]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0b013e31816b4acc"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119664"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5346"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600730"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.2979"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.19.3299"},{"key":"e_1_2_7_9_2","first-page":"154","volume-title":"Culturing hippocampal neurons and astrocytes from fetal brain. Cell biology: a laboratory handbook","author":"de Hoop M","year":"1998"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1088679"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199811)20:11<901::AID-BIES5>3.0.CO;2-0"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.4.605"},{"key":"e_1_2_7_13_2","first-page":"436","volume-title":"Myotonic dystrophy","author":"Harper PS","year":"2001"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0796-316"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:MCBI.0000009859.15267.db"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh327"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412845200"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2008.05.005"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090020501"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(03)00245-1"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.14.2167"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001134"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20800"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1103"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.hmg.a018923"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02283-9"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh266"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2005.06.009"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.12.1957"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880150212"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1128"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2004.03.012"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00504.2002"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.19.2143"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00023.2003"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5089"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5366"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.4.1402-1414.2005"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000065898.61358.09"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2007.05.032"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.16.5489-5501.2003"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012369462-1\/50006-5"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1159\/000072859"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2006.12.015"},{"key":"e_1_2_7_45_2","first-page":"109","article-title":"Postnatal growth and differentiation of muscle fibres in the mouse. I. A histochemical and morphometrical investigation of normal muscle","volume":"137","author":"Wirtz P","year":"1983","journal-title":"J Anat"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmus.21352","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.21352","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T02:08:05Z","timestamp":1697162885000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.21352"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,22]]},"references-count":44,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1002\/mus.21352"],"URL":"https:\/\/doi.org\/10.1002\/mus.21352","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,22]]}}}