{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T13:05:34Z","timestamp":1782997534922,"version":"3.54.5"},"reference-count":11,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,14]],"date-time":"2010-04-14T00:00:00Z","timestamp":1271203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study we describe the false\u2010positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage\u2010gated potassium channel (VGKC) autoantibodies, attributable to <jats:sup>125<\/jats:sup>I\u2010ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (<jats:italic>n<\/jats:italic> = 34,095) and VGKC autoantibody (<jats:italic>n<\/jats:italic> = 11,028). We retested sera that yielded apparently positive results with <jats:sup>125<\/jats:sup>1\u2010ligand with and without detergent\u2010solubilized cation\u2010channel protein, indentified clinically validated fals\u2010positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for <jats:sup>125<\/jats:sup>I\u2010ligand binding. Muscle Nerve, 2010<\/jats:p>","DOI":"10.1002\/mus.21653","type":"journal-article","created":{"date-parts":[[2010,4,14]],"date-time":"2010-04-14T23:11:54Z","timestamp":1271286714000},"page":"702-704","source":"Crossref","is-referenced-by-count":23,"title":["Eliminating false\u2010positive results in serum tests for neuromuscular autoimmunity"],"prefix":"10.1002","volume":"41","author":[{"given":"Metha","family":"Apiwattanakul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Mckeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean J.","family":"Pittock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Kryzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanda A.","family":"Lennon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,4,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.39.8.1069"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb51321.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10266"},{"key":"e_1_2_6_5_2","first-page":"1005","volume-title":"Manual of clinical and laboratory immunology","author":"Griesmann GE","year":"2002"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.6.554"},{"key":"e_1_2_6_7_2","first-page":"1013","volume-title":"Manual of clinical and laboratory immunology","author":"Vernino S","year":"2002"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000312275.04260.a0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.48.Suppl_5.23S"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.116848"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000129274.12702.92"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000188881.46043.44"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmus.21653","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.21653","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T01:42:09Z","timestamp":1696902129000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.21653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,14]]},"references-count":11,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1002\/mus.21653"],"URL":"https:\/\/doi.org\/10.1002\/mus.21653","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,14]]}}}