{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T02:07:53Z","timestamp":1783044473790,"version":"3.54.6"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,12,23]],"date-time":"2014-12-23T00:00:00Z","timestamp":1419292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005202","name":"Muscular Dystrophy Association","doi-asserted-by":"publisher","award":["176219"],"award-info":[{"award-number":["176219"]}],"id":[{"id":"10.13039\/100005202","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01NS051833"],"award-info":[{"award-number":["R01NS051833"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Introduction<\/jats:italic>: Lambert\u2013Eaton myasthenic syndrome (LEMS) is an autoimmune presynaptic neuromuscular disorder. Autoantibodies against subunits of voltage\u2010gated calcium channels (VGCCs) associated with acetylcholine release are thought to cause LEMS. <jats:italic>Methods<\/jats:italic>: HEK293 cells expressing specific individual recombinant subunits of \u03b1<jats:sub>1A<\/jats:sub>, \u03b1<jats:sub>1B<\/jats:sub>, \u03b1<jats:sub>1C<\/jats:sub>, and \u03b1<jats:sub>1E<\/jats:sub>; \u03b2<jats:sub>3<\/jats:sub>; and \u03b1<jats:sub>2<\/jats:sub>\u03b4 of human neuronal VGCCs were exposed to antibodies from 3 LEMS patients, 1 patient with small\u2010cell lung carcinoma, and 1 with myasthenia gravis. <jats:italic>Results<\/jats:italic>: All LEMS patient antibodies bound to cells containing any of the \u03b1<jats:sub>1<\/jats:sub> or \u03b2<jats:sub>3<\/jats:sub> subunits alone or combined with \u03b1<jats:sub>2<\/jats:sub>\u03b4 subunits, but not \u03b1<jats:sub>2<\/jats:sub>\u03b4 alone. Autoantibodies from the patient with small\u2010cell lung carcinoma but not the myasthenia gravis patient targeted the same VGCC subunits. <jats:italic>Conclusions<\/jats:italic>: Autoantibodies from LEMS patients bind directly to multiple VGCC \u03b1<jats:sub>1<\/jats:sub> subunits as well as the \u03b2<jats:sub>3<\/jats:sub> subunit. Thus, multiple components of the presynaptic VGCC complex are prospective targets for antibodies in LEMS. Muscle Nerve <jats:bold>51<\/jats:bold>: 176\u2013184, 2015<\/jats:p>","DOI":"10.1002\/mus.24295","type":"journal-article","created":{"date-parts":[[2014,5,24]],"date-time":"2014-05-24T09:54:55Z","timestamp":1400925295000},"page":"176-184","source":"Crossref","is-referenced-by-count":15,"title":["Lambert\u2013Eaton syndrome antibodies target multiple subunits of voltage\u2010gated Ca<sup>2+<\/sup> channels"],"prefix":"10.1002","volume":"51","author":[{"given":"Ravindra K.","family":"Hajela","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University B331 Life Sciences Building, 1355 Bogue Street East Lansing Michigan 48824\u20101317 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin M.","family":"Huntoon","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Michigan State University B331 Life Sciences Building, 1355 Bogue Street East Lansing Michigan 48824\u20101317 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