{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T17:18:50Z","timestamp":1784135930846,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T00:00:00Z","timestamp":1465430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In this study we examined the mechanisms of motor dysfunction in type 2 diabetes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Contractile force was measured in isolated nerve\u2013muscle preparations of db\/db mice using various protocols for electrical stimulation. Sarcoplasmic reticulum Ca<jats:sup>2+<\/jats:sup> adenosine triphosphatase protein (SERCA) was quantified by comparing Ca<jats:sup>2+<\/jats:sup>\u2010dependent and non\u2010specific phosphorylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with controls, the muscle\u2013nerve preparations of db\/db mice displayed muscle atrophy, reduced axonal excitability, and force deficit when stimulated via the nerve. Muscle relaxation after contraction was slowed, and SERCA content was reduced. In contrast, the sensitivity of the neuromuscular junction to tubocurarine and muscle fiber excitability were not affected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The force deficit in db\/db muscles was caused by atrophy and failure of neuromuscular signal transmission related to motor nerve axonal dysfunction. The slowed relaxation rate generally observed in diabetic muscles can, to a large extent, be explained by decreased SERCA pump content. <jats:italic>Muscle Nerve<\/jats:italic> <jats:bold>54<\/jats:bold>: 460\u2013468, 2016<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.25064","type":"journal-article","created":{"date-parts":[[2016,2,2]],"date-time":"2016-02-02T08:24:42Z","timestamp":1454401482000},"page":"460-468","source":"Crossref","is-referenced-by-count":30,"title":["Skeletal muscle dysfunction in the db\/db mouse model of type 2 diabetes"],"prefix":"10.1002","volume":"54","author":[{"given":"Jeppe Seamus","family":"Bayley","sequence":"first","affiliation":[{"name":"Department of Biomedicine Aarhus University Building 1163, Ole Worms All\u00e9 4 DK\u20108000 Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Holm","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Building 1163, Ole Worms All\u00e9 4 DK\u20108000 Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3535-7862","authenticated-orcid":false,"given":"Ole B\u00e6kgaard","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Building 1163, Ole Worms All\u00e9 4 DK\u20108000 Aarhus C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2009.10.007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.1.4.252"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2214\/AJR.09.2494"},{"key":"e_1_2_6_5_1","first-page":"1832\u20131836","article-title":"Chronic symmetric symptomatic polyneuropathy in the elderly: a field screening investigation in two Italian regions. 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