{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:21:32Z","timestamp":1774462892426,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,8,17]],"date-time":"2016-08-17T00:00:00Z","timestamp":1471392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Myasthenia Gravis Foundation of America, October 2015, Honolulu, Hawaii"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Introduction<\/jats:italic>: We investigated the effects of 3,4\u2010diaminopyridine (3,4\u2010DAP) and its acetylated metabolite, N\u2010(4\u2010amino\u2010pyridin\u20103\u2010yl) acetamide (3\u2010Ac), at the mammalian neuromuscular junction. <jats:italic>Methods<\/jats:italic>: Quantal release of acetylcholine was studied in diaphragm muscles of mice, using <jats:italic>in vitro<\/jats:italic> intracellular microelectrode recordings. <jats:italic>Results<\/jats:italic>: Under conditions of low probability of release, 3,4\u2010DAP produced a 1,000% increase in quantal release, but 3\u2010Ac had no effect. Under conditions of normal probability of release, the effect of 3,4\u2010DAP was modest and limited by concurrent depletion of synaptic vesicles, especially with high concentrations of 3,4\u2010DAP and high frequencies of nerve stimulation. <jats:italic>Conclusions<\/jats:italic>: These findings predict 3,4\u2010DAP is most effective in conditions with low probability of quantal release, such as Lambert\u2010Eaton myasthenic syndrome. A beneficial effect is also expected in disorders of neuromuscular transmission in which the effect of 3,4\u2010DAP on quantal release is not limited by depletion of synaptic vesicles, such as postsynaptic congenital myasthenic syndromes. <jats:italic>Muscle Nerve<\/jats:italic>, 2016 <jats:italic>Muscle Nerve<\/jats:italic> <jats:bold>55<\/jats:bold>: 223\u2013231, 2017<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.25208","type":"journal-article","created":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T08:39:02Z","timestamp":1464856742000},"page":"223-231","source":"Crossref","is-referenced-by-count":10,"title":["Effect of 3,4\u2010diaminopyridine at the murine neuromuscular junction"],"prefix":"10.1002","volume":"55","author":[{"given":"Fiona","family":"Ng","sequence":"first","affiliation":[{"name":"University of California, Davis School of Medicine Department of Neurology, University of California Davis Davis California 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