{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:12:54Z","timestamp":1773947574913,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,5,17]],"date-time":"2018-05-17T00:00:00Z","timestamp":1526515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007873","name":"Heimer-Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007873","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Protein Research Unit Ruhr within Europe, Ministry of Innovation, Science and Research of North-Rhine Westphalia","award":["233 - 1.08.03.03-031 -68079"],"award-info":[{"award-number":["233 - 1.08.03.03-031 -68079"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Introduction<\/jats:italic>: The aim of this study was the label\u2010free identification of distinct myopathological features with coherent anti\u2010Stokes Raman scattering (CARS) imaging, which leaves the sample intact for further analysis. <jats:italic>Methods<\/jats:italic>: The protein distribution was determined without labels by CARS at 2,930\u2009cm<jats:sup>\u22121<\/jats:sup> and was compared with the results of standard histological staining. <jats:italic>Results<\/jats:italic>: CARS imaging allowed the visualization of glycogen accumulation in glycogen storage disease type 5 (McArdle disease) and of internal nuclei in centronuclear myopathy. CARS identified an inhomogeneous protein distribution within muscle fibers in sporadic inclusion body myositis that was not shown with standard staining. In Duchenne muscular dystrophy, evidence for a higher protein content at the border of hypercontracted fibers was detected. <jats:italic>Discussion<\/jats:italic>: CARS enables the label\u2010free identification of distinct myopathological features, possibly paving the way for subsequent proteomic, metabolic, and genomic analyses. <jats:italic>Muscle Nerve<\/jats:italic> <jats:bold>58<\/jats:bold>: 457\u2013460, 2018<\/jats:p>","DOI":"10.1002\/mus.26140","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T01:00:26Z","timestamp":1523926826000},"page":"456-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Label\u2010free identification of myopathological features with coherent anti\u2010Stokes Raman scattering"],"prefix":"10.1002","volume":"58","author":[{"given":"Daniel","family":"Niedieker","sequence":"first","affiliation":[{"name":"Department of Biophysics Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederik","family":"Grosser\u00dcschkamp","sequence":"additional","affiliation":[{"name":"Department of Biophysics Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Schreiner","sequence":"additional","affiliation":[{"name":"Neurological Clinic Bergmannsheil Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Barkovits","sequence":"additional","affiliation":[{"name":"Medizinisches Proteom\u2010Center Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3599-9657","authenticated-orcid":false,"given":"Carsten","family":"K\u00f6tting","sequence":"additional","affiliation":[{"name":"Department of Biophysics Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrin","family":"Marcus","sequence":"additional","affiliation":[{"name":"Medizinisches Proteom\u2010Center Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Gerwert","sequence":"additional","affiliation":[{"name":"Department of Biophysics Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Vorgerd","sequence":"additional","affiliation":[{"name":"Neurological Clinic Bergmannsheil Ruhr\u2010University Bochum Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.anchem.1.031207.112754"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2520-x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2014.03.025"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5CS00564G"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.12.027"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1088\/1612-2011\/10\/2\/025604"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6AN02072K"},{"key":"e_1_2_7_9_1","first-page":"404","article-title":"Assessing the efficacy of coherent anti\u2010stokes raman scattering microscopy for the detection of infiltrating glioblastoma in fresh brain samples","volume":"414","author":"Galli R","year":"2016","journal-title":"J Biophotonics"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3534782"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.optcom.2007.07.067"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2009.195040"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24847"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.023176"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep44829"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2006.09.016"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018627705273"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmus.26140","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.26140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T06:44:45Z","timestamp":1694846685000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.26140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,17]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1002\/mus.26140"],"URL":"https:\/\/doi.org\/10.1002\/mus.26140","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,17]]},"assertion":[{"value":"2018-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}