{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T20:55:31Z","timestamp":1768078531264,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,8,23]],"date-time":"2018-08-23T00:00:00Z","timestamp":1534982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Introduction<\/jats:italic>: Antitumor immune responses are postulated to initiate paraneoplastic neurological disorders when proteins that are normally restricted to neural cells are expressed as oncoproteins. Mutated oncopeptides could bypass self\u2010tolerant T cells to activate cytotoxic effector T lymphocytes and requisite helper T lymphocytes to stimulate autoantibody production by B lymphocytes. <jats:italic>Methods<\/jats:italic>: We investigated muscle\u2010type nicotinic acetylcholine receptor (AChR) antigen expression at transcriptional and protein levels in a small\u2010cell lung cancer line (SCLC) established from a patient with AChR\u2010immunoglobulin G (IgG)\u2010positive myasthenia gravis. <jats:italic>Results<\/jats:italic>: We identified messenger RNA transcripts encoding the 2 AChR \u03b11\u2010subunit isoforms and 7 alternative\u2010splicing products, 3 of which yielded premature stop codons. Despite detecting native muscle\u2010type AChR pentamers in the tumor, we did not identify mutant \u03b11\u2010peptides. However, we found \u03b11\u2010subunit\u2010derived peptides bound to tumor major histocompatibility complex (MHC)1\u2010protein. In a control SCLC from an antineuronal nuclear autoantibody, type 1 (anti\u2010Hu)\u2010IgG\u2010positive patient, we identified MHC1\u2010complexed Hu protein\u2010derived peptides but not AChR peptides. <jats:italic>Discussion<\/jats:italic>: Our findings support onconeural protein products as pertinent immunogens initiating paraneoplastic neurological autoimmunity. <jats:italic>Muscle Nerve<\/jats:italic> <jats:bold>58<\/jats:bold>: 600\u2013604, 2018<\/jats:p>","DOI":"10.1002\/mus.26166","type":"journal-article","created":{"date-parts":[[2018,5,9]],"date-time":"2018-05-09T23:39:06Z","timestamp":1525909146000},"page":"600-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Mutated cancer autoantigen implicated cause of paraneoplastic myasthenia gravis"],"prefix":"10.1002","volume":"58","author":[{"given":"Anastasia","family":"Zekeridou","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic 200 First Street SW, Rochester, Minnesota 55905 USA"},{"name":"Department of Neurology Mayo Clinic Rochester Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy E.","family":"Griesmann","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic 200 First Street SW, Rochester, Minnesota 55905 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanda A.","family":"Lennon","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic 200 First Street SW, Rochester, Minnesota 55905 USA"},{"name":"Department of Neurology Mayo Clinic Rochester Minnesota USA"},{"name":"Department of Immunology Mayo Clinic Rochester Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.4049\/jimmunol.146.7.2245","article-title":"Recombinant human acetylcholine receptor \u03b1\u2010subunit induces chronic experimental autoimmune myasthenia gravis","volume":"146","author":"Lennon VA","year":"1991","journal-title":"J Immunol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2015.27"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2016.0603"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01144.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203486"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1246886"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69062302.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1998.tb10998.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/3.10.983"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17429"},{"key":"e_1_2_7_12_1","first-page":"271","article-title":"Quantitating defective ribosome products","volume":"301","author":"Qian S\u2010B","year":"2005","journal-title":"Methods Mol Biol"},{"key":"e_1_2_7_13_1","first-page":"6232","article-title":"Potentiation of interferon induction of class I major histocompatibility complex antigen expression by human tumor necrosis factor in small cell lung cancer cell ines","volume":"49","author":"Marley GM","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.126"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/305818a0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb07378.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.23.5463"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1645"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.21.4364"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.07.012"},{"key":"e_1_2_7_21_1","first-page":"2042","article-title":"Patients with lung cancer and paraneoplastic Hu syndrome harbor HuD\u2010specific type 2 CD8+ T cells","volume":"119","author":"Roberts WK","year":"2009","journal-title":"J Clin Invest"}],"container-title":["Muscle &amp; 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