{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T16:12:35Z","timestamp":1787933555553,"version":"build-2784847793"},"reference-count":73,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T00:00:00Z","timestamp":1535932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000945","name":"Pittsburgh Foundation","doi-asserted-by":"publisher","award":["AD2016\u20101765\u201029"],"award-info":[{"award-number":["AD2016\u20101765\u201029"]}],"id":[{"id":"10.13039\/100000945","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Duchenne muscular dystrophy (DMD) has been well characterized as a disease that affects both skeletal muscle and bone. The pathophysiology responsible for the deficits in bone tissue is still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative reverse\u2010transcription polymerase chain reaction and Western blot analyses of known myokines from skeletal muscle were performed on dystrophic mouse models and wild\u2010type (WT) controls to identify differentially expressed bone\u2010regulating myokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010four of 43 myokine genes demonstrated significantly different mRNA expression in the skeletal muscles of dystrophic mice when compared with muscles of WT mice. Several differently expressed bone\u2010regulating myokine genes were identified, and their protein levels were also verified by Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Dystrophic skeletal muscle demonstrated a significantly altered myokine gene expression profile. mRNA and protein levels of several bone\u2010regulating myokines were significantly altered in dystrophic skeletal muscle, which suggests pathological role of bone\u2010regulating myokines on bone homeostasis in DMD. <jats:italic>Muscle Nerve<\/jats:italic> <jats:bold>58<\/jats:bold>: 573\u2013582, 2018<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.26195","type":"journal-article","created":{"date-parts":[[2018,7,20]],"date-time":"2018-07-20T16:25:11Z","timestamp":1532103911000},"page":"573-582","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Altered bone\u2010regulating myokine expression in skeletal muscle Of Duchenne muscular dystrophy mouse models"],"prefix":"10.1002","volume":"58","author":[{"given":"Shumin","family":"Zhou","sequence":"first","affiliation":[{"name":"Musculoskeletal Growth &amp; Regeneration Laboratory, Department of Orthopaedic Surgery University of Pittsburgh  Pittsburgh Pennsylvania, 15219 USA"},{"name":"Department of Orthopaedic Surgery Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai, 200233 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoli","family":"Qian","sequence":"additional","affiliation":[{"name":"Musculoskeletal Growth &amp; Regeneration Laboratory, Department of Orthopaedic Surgery University of Pittsburgh  Pittsburgh Pennsylvania, 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wang","sequence":"additional","affiliation":[{"name":"Vascular Medicine Institute, Department of Medicine University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai, 200233 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Macalus V.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Musculoskeletal Growth &amp; Regeneration Laboratory, Department of Orthopaedic Surgery University of Pittsburgh  Pittsburgh Pennsylvania, 15219 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9793-0540","authenticated-orcid":false,"given":"Hongshuai","family":"Li","sequence":"additional","affiliation":[{"name":"Musculoskeletal Growth &amp; Regeneration Laboratory, Department of Orthopaedic Surgery University of Pittsburgh  Pittsburgh Pennsylvania, 15219 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