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It is poorly understood how long\u2010term changes in cholinergic transmission contribute to age\u2010 and disease\u2010related degeneration in the motor system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study we performed confocal imaging, electrophysiology, electron microscopy, and analyses of respiratory mechanics of the diaphragm NMJ components in 12\u2010month\u2010old wild\u2010type (WT) and VAChTKD<jats:sup>HOM<\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Diaphragms of NMJs of the VAChTKD<jats:sup>HOM<\/jats:sup> mice were similar to those in WT mice in number, colocalization, and fragmentation of pre\u2212\/postsynaptic components. However, they had increased spontaneous SV exocytosis, miniature endplate potential frequency, and diminished MEPP amplitude. No impairment in respiratory mechanics at rest was observed, probably due to the large neurotransmission safety factor of the diaphragm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The present findings help us to understand the consequences of reduced ACh release at the NMJs during aging.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.26710","type":"journal-article","created":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T07:14:59Z","timestamp":1568790899000},"page":"790-800","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the neuromuscular junction in a middle\u2010aged mouse model of congenital myasthenic syndrome"],"prefix":"10.1002","volume":"60","author":[{"given":"Luana Pereira","family":"Leite Schetino","sequence":"first","affiliation":[{"name":"Faculdade de Medicina Universidade Federal dos Vales do Jequitinhonha e Mucuri  Diamantina Minas Gerais 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Camargo","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermann Alecsandro","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias B\u00e1sicas da Vida, Instituto de Ci\u00eancias da Vida Universidade Federal de Juiz de Fora  Campus Governador Valadares Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Neves","family":"Andrade","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia Universidade Federal de Minas Gerais  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Magalh\u00e3es","family":"Arantes\u2010Costa","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Escola de Medicina Universidade de S\u00e3o Paulo  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