{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T18:06:27Z","timestamp":1775066787631,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T00:00:00Z","timestamp":1573171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Research Unit 1228"],"award-info":[{"award-number":["Research Unit 1228"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006254","name":"Ruhr-Universit\u00e4t Bochum","doi-asserted-by":"publisher","award":["FoRUM F755\u201012"],"award-info":[{"award-number":["FoRUM F755\u201012"]}],"id":[{"id":"10.13039\/501100006254","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Sporadic inclusion body myositis (sIBM) is characterized by myopathological features including rimmed vacuoles (RVs) and proteins associated with protein aggregation, autophagy, and inflammation. Previous proteomic studies of RV areas revealed an overrepresentation of several chaperones and subunits of the T\u2010complex protein 1 (TCP\u20101), which is involved in prevention of protein aggregation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To validate our proteomic findings, immunofluorescence analyses of selected chaperones and quantitative Western blot analysis of TCP\u20101 proteins were performed in five sIBM patients and five healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunofluorescence studies confirmed increased immunoreactivity for VCP, UNC45B, GRP\u201075, \u03b1B\u2010crystallin, LAMP\u20102, Rab\u20107a, and TCP\u20101\u03b1 and TCP\u2010\u03b8 in RVs. Quantitative Western blot analysis revealed a significantly higher level of TCP\u20101 in sIBM muscle tissue when compared with healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Our study findings validate new insights in protein quality control and degradation processes that seem to be relevant in sIBM. These data provide an important basis for future functional and therapeutic studies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.26742","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T19:33:35Z","timestamp":1571859215000},"page":"116-121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Chaperones in sporadic inclusion body myositis\u2014Validation of proteomic data"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4017-940X","authenticated-orcid":false,"given":"Anne\u2010Katrin","family":"G\u00fcttsches","sequence":"first","affiliation":[{"name":"Department of Neurology Heimer Institute for Muscle Research, University Hospital Bergmannsheil, Ruhr University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Neurology Heimer Institute for Muscle Research, University Hospital Bergmannsheil, Ruhr University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Schreiner","sequence":"additional","affiliation":[{"name":"Department of Neurology Heimer Institute for Muscle Research, University Hospital Bergmannsheil, Ruhr University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janine","family":"Mertens\u2010Rill","sequence":"additional","affiliation":[{"name":"Department of Neurology Heimer Institute for Muscle Research, University Hospital Bergmannsheil, Ruhr University Bochum  Bochum Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Tegenthoff","sequence":"additional","affiliation":[{"name":"Department of Neurology Heimer Institute for Muscle Research, University Hospital Bergmannsheil, Ruhr University Bochum  Bochum 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