{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T20:08:13Z","timestamp":1773346093639,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54NS065736"],"award-info":[{"award-number":["U54NS065736"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54NS065736"],"award-info":[{"award-number":["U54NS065736"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>To develop a new method to quantify the density of nerves, vessels, and the neurovascular contacts, we studied skin biopsies in diabetes and control subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Skin biopsies with dual immunofluorescent staining were used to visualize nerves and blood vessels. The density of nerves, vessels, and their neurovascular contacts were quantified with unbiased stereology. Results were compared with examination findings, validated questionnaires, and autonomic function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In tissue from 19 controls and 20 patients with diabetes, inter\u2010rater and intra\u2010rater intraclass correlation coefficients were high (&gt;0.85; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001) for all quantitative methods. In diabetes, the nerve densities (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05), vessel densities (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01), and the neurovascular densities (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01) were lower compared with 20 controls. Results correlated with autonomic function, examination and symptom scores.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>We report an unbiased, stereological method to quantify the cutaneous nerve, vessel and neurovascular density and offer new avenues of investigation into cutaneous neurovascular innervation in health and disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.26889","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T06:22:44Z","timestamp":1586413364000},"page":"492-501","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A novel method to quantify cutaneous vascular innervation"],"prefix":"10.1002","volume":"62","author":[{"given":"Eunhee","family":"Sohn","sequence":"first","affiliation":[{"name":"Department of Neurology Chungnam University Hospital  Daejeon South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3947-5615","authenticated-orcid":false,"given":"Bum Chun","family":"Suh","sequence":"additional","affiliation":[{"name":"Department of Neurology Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningshan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Beth Israel Deaconess Medical Center Harvard Medical School  Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy","family":"Freeman","sequence":"additional","affiliation":[{"name":"Department of Neurology, Beth Israel Deaconess Medical Center Harvard Medical School  Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher H.","family":"Gibbons","sequence":"additional","affiliation":[{"name":"Department of Neurology, Beth Israel 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