{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T20:36:45Z","timestamp":1781123805413,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T00:00:00Z","timestamp":1588896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS071067"],"award-info":[{"award-number":["R01NS071067"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007229","name":"Harvard University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007229","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Conventional processing of nerve for histomorphometry is resource\u2010intensive, precluding use in intraoperative assessment of nerve quality during nerve transfer procedures. Stimulated Raman scattering (SRS) microscopy is a label\u2010free technique that enables rapid and high\u2010resolution histology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Segments of healthy murine sciatic nerve, healthy human obturator nerve, and human cross\u2010facial nerve autografts were imaged on a custom SRS microscope. Myelinated axon quantification was performed through segmentation using a random forest machine learning algorithm in commercial software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High contrast, high\u2010resolution imaging of nerve morphology was obtained with SRS imaging. Automated myelinated axon quantification from cross\u2010sections of healthy human nerve imaged using SRS was achieved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Herein, we demonstrate the use of a label\u2010free technique for rapid imaging of murine and human peripheral nerve cryosections. We illustrate the potential of this technique to inform intraoperative decision\u2010making through rapid automated quantification of myelinated axons using a machine learning algorithm.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.26895","type":"journal-article","created":{"date-parts":[[2020,4,18]],"date-time":"2020-04-18T09:30:40Z","timestamp":1587202240000},"page":"137-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Label\u2010free histomorphometry of peripheral nerve by stimulated Raman spectroscopy"],"prefix":"10.1002","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3611-7959","authenticated-orcid":false,"given":"Iv\u00e1n","family":"Coto Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Massachusetts Eye and Ear, Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlong","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Advanced Imaging Harvard University  Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Mohan","sequence":"additional","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Massachusetts Eye and Ear, Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nate","family":"Jowett","sequence":"additional","affiliation":[{"name":"Surgical Photonics and Engineering Laboratory Massachusetts Eye and Ear, Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2018.07.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199804233381702"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2018-312563"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e3181babb93"},{"key":"e_1_2_9_6_1","article-title":"Toluidine blue staining of resin\u2010embedded sections for evaluation of peripheral nerve morphology","volume":"137","author":"Ghnenis AB","year":"2018","journal-title":"J Vis Exp."},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2007.06.018"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000005338"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/nlz099"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417820111"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3495"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1165758"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3638180"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3533312"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13283"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e318191c5b8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.32655"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/119.1.271"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-016-0027"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.5494"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.10.004329"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.7.076019"}],"container-title":["Muscle &amp; 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