{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T12:46:59Z","timestamp":1782996419909,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HA 3309\/3\u20101"],"award-info":[{"award-number":["HA 3309\/3\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The muscle relaxant methocarbamol is widely used for the treatment of muscle spasms and pain syndromes. To elucidate molecular mechanisms of its action, we studied its influence on neuromuscular transmission, on isometric muscle force, and on voltage\u2010gated Na<jats:sup>+<\/jats:sup>channels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Neuromuscular transmission was investigated in murine diaphragm\u2010phrenic nerve preparations and muscle force studied on mouse soleus muscles. Na<jats:sub>v<\/jats:sub>1.4 channels and Na<jats:sub>v<\/jats:sub>1.7 channels were functionally expressed in eukaryotic cell lines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Methocarbamol, at 2\u2009mM, decreased the decay of endplate currents, slowed the decay of endplate potentials and reduced tetanic force of soleus muscles. The drug reversibly inhibited current flow through muscular Na<jats:sub>v<\/jats:sub>1.4 channels, while neuronal Na<jats:sub>v<\/jats:sub>1.7 channels were unaffected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The study provides evidence for peripheral actions of methocarbamol on skeletal muscle. Muscular Na<jats:sup>+<\/jats:sup>channels are a molecular target of methocarbamol. Since Na<jats:sub>v<\/jats:sub>1.7 currents were unaffected, methocarbamol is unlikely to exert its analgesic effect by directly blocking Na<jats:sub>v<\/jats:sub>1.7 channels.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.27087","type":"journal-article","created":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T03:34:45Z","timestamp":1602473685000},"page":"141-150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Methocarbamol blocks muscular Na<sub>v<\/sub>1.4 channels and decreases isometric force of mouse muscles"],"prefix":"10.1002","volume":"63","author":[{"given":"Yaxin","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Pathophysiology University Medicine Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Otto","sequence":"additional","affiliation":[{"name":"Institute of Pathophysiology University Medicine Greifswald 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