{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:12:49Z","timestamp":1790190769333,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T00:00:00Z","timestamp":1608768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["K23NS085049"],"award-info":[{"award-number":["K23NS085049"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The immunopathology of autoimmune seronegative myasthenia gravis (SN MG) is poorly understood. Our objective was to determine immune profiles associated with a diagnosis of SN MG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed high\u2010dimensional flow cytometry on blood samples from SN MG patients (N = 68), healthy controls (N = 46), and acetylcholine receptor antibody (AChR+) MG patients (N = 27). We compared 12 immune cell subsets in SN MG to controls using logistic modeling via a discovery\u2010replication design. An exploratory analysis fit a multinomial model comparing AChR+ MG and controls to SN MG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>An increase in CD19<jats:sup>+<\/jats:sup>CD20<jats:sup>\u2212<\/jats:sup>CD38<jats:sup>hi<\/jats:sup>plasmablast frequencies was associated with lower odds of being a SN MG case in both the discovery and replication analyses (discovery<jats:italic>P<\/jats:italic>\u2010value = .0003, replication<jats:italic>P<\/jats:italic>\u2010value = .0021). Interleukin (IL) \u201021 producing helper T cell frequencies were associated with a diagnosis of AChR+ MG (<jats:italic>P<\/jats:italic>=\u2009.004).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Reduced plasmablast frequencies are strongly associated with a SN MG diagnosis and may be a useful diagnostic biomarker in the future.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.27140","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T05:42:37Z","timestamp":1607578957000},"page":"577-585","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Reduced plasmablast frequency is associated with seronegative myasthenia gravis"],"prefix":"10.1002","volume":"63","author":[{"given":"Jeffrey T.","family":"Guptill","sequence":"first","affiliation":[{"name":"Neuromuscular Division, Department of Neurology Duke University Medical Center Durham North Carolina 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