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In myotonic dystrophy type 1 (DM1), the RBP MBNL1 (muscleblind\u2010like) is sequestered by toxic CUG repeats, leading to missplicing of MBNL1 targets. Mounting evidence from the literature has implicated other factors in the pathogenesis of DM1. Herein we sought to evaluate the functional role of the splicing factor hnRNP L in normal and DM1 muscle cells.<\/jats:p><\/jats:sec>Methods<\/jats:title>Co\u2010immunoprecipitation assays using hnRNPL and MBNL1 expression constructs and splicing profiling in normal and DM1 muscle cell lines were performed. Zebrafish morpholinos targeting hnrpl<\/jats:italic> and hnrnpl2<\/jats:italic> were injected into one\u2010cell zebrafish for developmental and muscle analysis. In human myoblasts downregulation of hnRNP L was achieved with shRNAi. Ascochlorin administration to DM1 myoblasts was performed and expression of the CUG repeats, DM1 splicing biomarkers, and hnRNP L expression levels were evaluated.<\/jats:p><\/jats:sec>Results<\/jats:title>Using DM1 patient myoblast cell lines we observed the formation of abnormal hnRNP L nuclear foci within and outside the expanded CUG repeats, suggesting a role for this factor in DM1 pathology. We showed that the antiviral and antitumorigenic isoprenoid compound ascochlorin increased MBNL1 and hnRNP L expression levels. Drug treatment of DM1 muscle cells with ascochlorin partially rescued missplicing of established early biomarkers of DM1 and improved the defective myotube formation displayed by DM1 muscle cells.<\/jats:p><\/jats:sec>Discussion<\/jats:title>Together, these studies revealed that hnRNP L can modulate DM1 pathologies and is a potential therapeutic target.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.27216","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T15:48:30Z","timestamp":1614700110000},"page":"928-940","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["hnRNP L<\/scp> is essential for myogenic differentiation and modulates myotonic dystrophy pathologies"],"prefix":"10.1002","volume":"63","author":[{"given":"Matthew S.","family":"Alexander","sequence":"first","affiliation":[{"name":"Division of Neurology, Department of Pediatrics University of Alabama at Birmingham and Children's of Alabama Birmingham Alabama USA"},{"name":"Center for Exercise Medicine University of Alabama at Birmingham Birmingham Alabama USA"},{"name":"Department of Genetics University of Alabama at Birmingham Birmingham Alabama USA"},{"name":"Civitan International Research Center University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rylie M.","family":"Hightower","sequence":"additional","affiliation":[{"name":"Division of Neurology, Department of Pediatrics University of Alabama at Birmingham and Children's of Alabama Birmingham Alabama USA"},{"name":"Center for Exercise Medicine University of Alabama at Birmingham Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea L.","family":"Reid","sequence":"additional","affiliation":[{"name":"Division of Neurology, Department of Pediatrics University of Alabama at Birmingham and Children's of Alabama Birmingham Alabama 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