{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:31:30Z","timestamp":1793316690964,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T00:00:00Z","timestamp":1717977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction\/Aims<\/jats:title><jats:p>The circulating microRNAs (miRNAs) miR\u2010150\u20105p, miR\u201030e\u20105p, and miR\u201021\u20105p have been suggested as potential biomarkers for myasthenia gravis (MG); however, the relationships between short\u2010term natural changes of the miRNAs and patient\u2010reported MG outcome scores have not been well\u2010studied. We assessed the short\u2010term fluctuations in miRNA levels and patient\u2010reported outcome measures in MG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This prospective cohort study included 39 MG patients with regular follow\u2010ups and unchanged medications at the Neurology outpatient clinic at Uppsala University Hospital. Patients had weekly follow\u2010up visits for 1\u2009month, at which blood samples were drawn, and scores from MG activities of daily living (MG\u2010ADL), MG quality\u2010of\u2010life\u201015 (MG\u2010QoL15), and Fatigue Severity Scale (FSS) were assessed. Serum levels of miRNA miR\u2010150\u20105p, miR\u201030e\u20105p, and miR\u201021\u20105p were analyzed using quantitative real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Intra\u2010individual levels of miR\u201030e\u20105p and miR\u2010150\u20105p were stable, whereas a significant reduction in miR\u201021\u20105p was observed from week 1 to week 2 (<jats:italic>p<\/jats:italic>\u2009=\u2009.0024) and from week 2 to week 3 (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.0001). There were intra\u2010individual differences over a short time in MG\u2010ADL, with higher scores in female patients (<jats:italic>p<\/jats:italic>\u2009=\u2009.0281) and a significant reduction from the first to the second weeks (<jats:italic>p<\/jats:italic>\u2009=\u2009.0281), whereas MG\u2010QoL15 and FSS scores were stable.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The suggested MG biomarkers miR\u201030e\u20105p and miR\u2010150\u20105p were more stable than miR\u201021\u20105p over a short time, indicating their short\u2010term stability as biomarkers. Prospective multi\u2010center studies with longer periods of follow\u2010up and matched controls are needed to validate these miRNAs as biomarkers in MG.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mus.28177","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T09:58:52Z","timestamp":1718013532000},"page":"284-289","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Short\u2010term changes in serum <scp>miRNA<\/scp> levels and patient\u2010reported clinical outcomes in myasthenia gravis"],"prefix":"10.1002","volume":"70","author":[{"given":"Yu\u2010Fang","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Medical Sciences, Clinical Neurophysiology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7116-0939","authenticated-orcid":false,"given":"Amol K.","family":"Bhandage","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Clinical Neurophysiology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa Diaz\u2010Pintado","family":"Adestr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Clinical Neurophysiology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Rostedt","family":"Punga","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Clinical Neurophysiology Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00463-4"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.52.7.1487"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21053"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/CND.0000000000000321"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00213"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11040740"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000450"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2015.06.019"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.24"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2018.05.003"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12883-023-03406-0"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0000000000002864"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00773"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00159-9"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.27476"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.26469"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2010.0392"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000207278"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.28177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T14:01:43Z","timestamp":1737468103000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.28177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,10]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/mus.28177"],"URL":"https:\/\/doi.org\/10.1002\/mus.28177","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,10]]},"assertion":[{"value":"2023-11-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}