{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:22:46Z","timestamp":1790270566177,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008333","name":"University of Alabama at Birmingham","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008333","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction\/Aims<\/jats:title>\n                    <jats:p>The precise relationship between molecular mimicry and tissue\u2010specific autoimmunity is unknown. Major histocompatibility complex (MHC) class II antigen presenting cell\u2010CD4+ T\u2010cell receptor complex interactions are necessary for adaptive immunity. This study aimed to determine the role of endoneurial endothelial cell MHC class II in autoimmune polyneuropathy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Cryopreserved Guillain\u2013Barr\u00e9 syndrome (GBS) patient sural nerve biopsies and sciatic nerves from the severe murine experimental autoimmune neuritis (sm\u2010EAN) GBS model were studied. Cultured conditional ready MHC Class II antigen A\u2010alpha chain (H2\u2010Aa) embryonic stem cells were used to generate H2\u2010Aa\n                      <jats:sup>flox\/+<\/jats:sup>\n                      C57BL\/6 mice. Mice were backcrossed and intercrossed to the SJL background to generate H2\u2010Aa\n                      <jats:sup>flox\/flox<\/jats:sup>\n                      SJL mice, bred with hemizygous Tamoxifen\u2010inducible von Willebrand factor Cre recombinase (vWF\u2010iCre\/+) SJL mice to generate H2\u2010Aa\n                      <jats:sup>flox\/flox<\/jats:sup>\n                      ; vWF\u2010iCre\/+ mice to study microvascular endothelial cell adaptive immune responses. Sm\u2010EAN was induced in Tamoxifen\u2010treated H2\u2010Aa\n                      <jats:sup>flox\/flox<\/jats:sup>\n                      ; vWF\u2010iCre\/+, H2\u2010Aa\n                      <jats:sup>flox\/flox<\/jats:sup>\n                      ; +\/+, H2\u2010Aa\n                      <jats:sup>+\/+<\/jats:sup>\n                      ; vWF\u2010iCre\/+ and untreated H2\u2010Aa\n                      <jats:sup>flox\/flox<\/jats:sup>\n                      ; vWF\u2010iCre\/+ adult female SJL mice. Neurobehavioral, electrophysiological and histopathological assessments were performed at predefined time points.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Endoneurial endothelial cell MHC class II expression was observed in normal and inflamed human and mouse peripheral nerves. Tamoxifen\u2010treated H2\u2010Aa\n                      <jats:sup>flox\/flox<\/jats:sup>\n                      ; vWF\u2010iCre\/+ mice were resistant to sm\u2010EAN despite extensive MHC class II expression in lymphoid and non\u2010lymphoid tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>A conditional MHC class II knockout mouse to study cell\u2010 and time\u2010dependent adaptive immune responses in vivo was developed. Initial studies show microvascular endothelial cell MHC class II expression is necessary for peripheral nerve specific autoimmunity, as advocated by human in vitro adaptive immunity and ex vivo transplant rejection studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mus.28193","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T09:08:11Z","timestamp":1719392891000},"page":"420-433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of a major histocompatibility complex class\n                    <scp>II<\/scp>\n                    conditional knockout mouse to study cell\u2010specific and time\u2010dependent adaptive immune responses in peripheral nerves"],"prefix":"10.1002","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8307-9994","authenticated-orcid":false,"given":"Eroboghene E.","family":"Ubogu","sequence":"first","affiliation":[{"name":"Neuromuscular Immunopathology Research Laboratory, Division of Neuromuscular Disease, Department of Neurology University of Alabama  Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy A.","family":"Conner","sequence":"additional","affiliation":[{"name":"Neuromuscular Immunopathology Research Laboratory, Division of Neuromuscular Disease, Department of Neurology University of Alabama  Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimin","family":"Wang","sequence":"additional","affiliation":[{"name":"Neuromuscular Immunopathology Research Laboratory, Division of Neuromuscular Disease, Department of Neurology University of Alabama  Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Yadav","sequence":"additional","affiliation":[{"name":"Neuromuscular Immunopathology Research Laboratory, Division of Neuromuscular Disease, Department of Neurology University of Alabama  Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas L.","family":"Saunders","sequence":"additional","affiliation":[{"name":"Transgenic Animal Model Core, University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2018.10.012"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2014.121"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00339-1"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aww247"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880150506"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410090713"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(99)00328-7"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199604)19:4<474::AID-MUS8>3.0.CO;2-9"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1599-0"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1466-4"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2156"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2007.00067.x"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00117-0"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130224"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.12.033"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01907"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2496"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.09.020"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002835"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.12.8386"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-5-5"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052586"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.13149"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1016361"},{"issue":"1","key":"e_1_2_12_26_1","article-title":"Blocking MHC class II on human endothelium mitigates acute rejection. 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