{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T07:26:29Z","timestamp":1764228389470,"version":"3.46.0"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:00:00Z","timestamp":1756080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005615","name":"Beth Israel Deaconess Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005615","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction\/Aims<\/jats:title>\n                    <jats:p>The compound motor action potential (CMAP) is a very well\u2010established output from standard motor conduction studies in patients. CMAP methods have also been developed for various animal models, including mice, rats, and dogs. Here, we describe a CMAP methodology for adult zebrafish.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using needle stimulating electrodes placed in proximity to the caudal spinal column and a fixed two\u2010electrode surface array placed near the dorsal fin for recording, we obtained CMAPs in wildtype (WT) and symptomatic amyotrophic lateral sclerosis (ALS) SOD1\n                      <jats:sup>G93A<\/jats:sup>\n                      zebrafish, assessing repeatability and the potential for identifying differences between the groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In WT animals, CMAP amplitude exhibited robust performance with a test\u2013retest intra\u2010class coefficient of 0.97 (95% confidence interval 0.947\u20130.988;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200930). SOD1\n                      <jats:sup>G93A<\/jats:sup>\n                      zebrafish exhibited a 36% lower supramaximal CMAP amplitude as compared to WT (mean\u2009\u00b1\u2009standard deviation: 7.7\u2009\u00b1\u20091.7\u2009mV versus 12.2\u2009\u00b1\u20091.8\u2009mV, respectively,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001) and an 11% longer latency (1.30\u2009\u00b1\u20090.15\u2009ms versus 1.17\u2009\u00b1\u20090.11\u2009ms,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002). A classifier, incorporating amplitude and latency together, provided perfect discrimination between the two cohorts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>CMAP recording is a reliable technique in zebrafish and can successfully differentiate healthy WT fish from ALS\u2010affected animals. Since CMAP is a quantitative metric that is highly sensitive to motor neuron loss or dysfunction, it will allow the zebrafish to be more effectively harnessed for physiological and clinical therapeutic studies in ALS and other neuromuscular diseases for which adult zebrafish models are available.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mus.70009","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T10:26:39Z","timestamp":1756117599000},"page":"1168-1177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Performing Compound Motor Action Potential Measurement in Zebrafish: A Description of Methodology and a Comparison Between Healthy and\n                    <scp>ALS<\/scp>\n                    \u2010Affected Animals"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6375-3312","authenticated-orcid":false,"given":"Seward","family":"Rutkove","sequence":"first","affiliation":[{"name":"Department of Neurology Beth Israel Deaconess Medical Center  Boston Massachusetts USA"},{"name":"Harvard Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2540-6005","authenticated-orcid":false,"given":"Gaurav","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Cardiology Beth Israel Deaconess Medical Center  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5032-7250","authenticated-orcid":false,"given":"Anjali K.","family":"Nath","sequence":"additional","affiliation":[{"name":"Harvard Medical School  Boston Massachusetts USA"},{"name":"Department of Cardiology Beth Israel Deaconess Medical Center  Boston Massachusetts USA"},{"name":"Broad Institute  Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,25]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnp.2024.04.002"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2020.135488"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.00195"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2018.04.748"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.11.022"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2022.02.020"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.24237"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395\u2010024\u201000349\u2010z"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99081"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinph.2011.01.045"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2018.08.003"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.029058"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2021.136041"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.015719"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750\u20101326\u20107\u201044"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.1.87"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1603471"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002517-199504000-00011"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008506\u2010199601000\u201000011"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000539\u2010199602000\u201000018"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201034119\u20106"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11071938"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcm.2016.02.012"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201096122\u2010z"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.70009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T07:20:27Z","timestamp":1764228027000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.70009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,25]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/mus.70009"],"URL":"https:\/\/doi.org\/10.1002\/mus.70009","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"type":"print","value":"0148-639X"},{"type":"electronic","value":"1097-4598"}],"subject":[],"published":{"date-parts":[[2025,8,25]]},"assertion":[{"value":"2025-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}