{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:50:36Z","timestamp":1786978236983,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100013465","name":"French Muscular Dystrophy Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013465","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction\/Aims<\/jats:title>\n                    <jats:p>\n                      In dystrophic mice (\n                      <jats:italic>mdx<\/jats:italic>\n                      , a genetic homolog of Duchenne muscular dystrophy: DMD), previous studies showed that mechanical ventilation (MV) induces ventilator\u2010induced diaphragmatic dysfunction (VIDD). However, susceptibility to mechanical stress caused by asynchrony remains unknown. Our aims were to investigate whether MV exacerbates diaphragm vulnerability to eccentric stress and to evaluate the role of RyR1 remodeling and NOX2 activation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male\n                      <jats:italic>mdx<\/jats:italic>\n                      and wild\u2010type (WT) mice (10\u201312\u2009weeks) were assigned to non\u2010ventilated or 6\u2010h MV groups. Diaphragm strength and susceptibility to eccentric contractions mimicking asynchrony were assessed, along with remodeling of the Ca\n                      <jats:sup>2+<\/jats:sup>\n                      release channel RyR1. Two preventive strategies were tested: in\u00a0vivo S107 treatment (RyR1\u2010stabilizing compound) administered before MV; and in\u00a0vitro incubation of diaphragms previously ventilated with S107 or ebselen (NOX2 inhibitor) just before eccentric stress.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MV reduced maximal tetanic force by 20.1% in WT and 27.0% in\n                      <jats:italic>mdx<\/jats:italic>\n                      mice without sarcolemmal injury. In\n                      <jats:italic>mdx<\/jats:italic>\n                      mice MV significantly increased susceptibility to eccentric contraction, causing a 45% greater force deficit and increased sarcolemmal damage. Pretreatment with S107 prevented both MV\u2010induced weakness and eccentric stress susceptibility. After 6\u2009h MV, incubation with S107 or ebselen mitigated susceptibility to eccentric contraction. RyR1 oxidation, phosphorylation, and calstabin1 dissociation induced by MV were exacerbated by eccentric stress; S107 preserved calstabin1 binding, while ebselen reduced RyR1 oxidative modifications.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>MV exacerbates diaphragm vulnerability to mechanical stress in dystrophin\u2010deficient muscle through RyR1 remodeling and NOX2 activation. Preventing asynchrony and targeting RyR1 or NOX2 may represent therapeutic strategies to limit respiratory complications in DMD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mus.70253","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:24:27Z","timestamp":1777101867000},"page":"255-264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Asynchrony Related to Mechanical Ventilation Exacerbates Diaphragm Dysfunction in a Mouse Model of Duchenne Muscular Dystrophy"],"prefix":"10.1002","volume":"74","author":[{"given":"Mohamad","family":"Yehya","sequence":"first","affiliation":[{"name":"PhyMedExp Montpellier University, INSERM, CNRS, CHRU Montpellier  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haikel","family":"Dridi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cellular Biophysics Clyde and Helen Wu Center for Molecular Cardiology Columbia University College of Physicians and Surgeons  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4548-9140","authenticated-orcid":false,"given":"Eric","family":"Est\u00e8ve","sequence":"additional","affiliation":[{"name":"PhyMedExp Montpellier University, INSERM, CNRS, CHRU Montpellier  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Moreau","sequence":"additional","affiliation":[{"name":"PhyMedExp Montpellier University, INSERM, CNRS, CHRU Montpellier  Montpellier France"},{"name":"Physiological Department Montpellier University and Montpellier University Health Care Center  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Reiken","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cellular Biophysics Clyde and Helen Wu Center for Molecular Cardiology Columbia University College of Physicians and Surgeons  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Marks","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cellular Biophysics Clyde and Helen Wu Center for Molecular Cardiology Columbia University College of Physicians and Surgeons  New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Lacampagne","sequence":"additional","affiliation":[{"name":"PhyMedExp Montpellier University, INSERM, CNRS, CHRU Montpellier  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1878-0936","authenticated-orcid":false,"given":"Stefan","family":"Matecki","sequence":"additional","affiliation":[{"name":"PhyMedExp Montpellier University, INSERM, CNRS, CHRU Montpellier  Montpellier France"},{"name":"Physiological Department Montpellier University and Montpellier University Health Care Center  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)93058-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8966(02)00140-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2007.03.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8966(03)00162-7"},{"key":"e_1_2_10_6_1","first-page":"643","article-title":"Effect of Intermittent Positive Pressure Ventilation on Life\u2010Span and Causes of Death in Duchenne Muscular Dystrophy","volume":"45","author":"Konagaya M.","year":"2005","journal-title":"Rinsh\u014d Shinkeigaku"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.105.2.445"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2004.03.028"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-2952J"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.20356"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200304-489CP"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa070447"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00591.2011"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00231.2013"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200202-088OC"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.11.025"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11978-4"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/pnasnexus\/pgad336"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.11.019"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.25760"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1609707113"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-006-0301-8"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/PCC.0b013e3182917922"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2012.04.015"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5862"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-023-03502-5"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e318261e7f8"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00117.2004"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3710"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00146.2004"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094301"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711074105"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2013.06.026"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0316295"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/352536a0"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP283860"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3040649"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006868130002"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1916"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.05.014"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-1083fje"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.280.2.C352"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203654200"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.1.C260"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112410200"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.165.3.2103028"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015354"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600451200"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0670-2"}],"container-title":["Muscle &amp; Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.70253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mus.70253","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.70253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T05:03:39Z","timestamp":1781931819000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.70253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,25]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/mus.70253"],"URL":"https:\/\/doi.org\/10.1002\/mus.70253","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,25]]},"assertion":[{"value":"2025-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}