{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:01:11Z","timestamp":1791392471972,"version":"4.3.3"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction\/Aims<\/jats:title>\n                    <jats:p>In amyotrophic lateral sclerosis (ALS), SIMOA\u2010based studies show that baseline serum neurofilament light chain (NfL) predicts the revised ALS Functional Rating Scale (ALSFRS\u2010R) decline rate and survival. The Roche Elecsys electrochemiluminescence immunoassay (ECLIA) reports values approximately six\u2010fold lower; cross\u2010platform comparison confirms comparable performance, but serial clinical\u2010practice data remain limited. We assessed whether ECLIA NfL correlates with the ALSFRS\u2010R decline rate under the sampling conditions of clinical practice, and whether GFAP or S\u2010100B adds prognostic or disease\u2010specific information.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We retrospectively analyzed 58 patients with medical\u2010record\u2010confirmed ALS at an academic center (2022\u20132026). Serum NfL, GFAP, and S\u2010100B were measured on the Roche Elecsys ECLIA. The NfL\u2010ALSFRS\u2010R correlation was assessed within matching windows; serial NfL was examined in patients with repeat draws.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      First\u2010per\u2010patient NfL median was 7.06\u2009pg\/mL (IQR 4.06\u201317.30). NfL correlated strongly with the ALSFRS\u2010R decline\u00a0rate (Spearman\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.704,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200931;\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.809 within 90\u2009days,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200917; both\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Fast progressors had 3.7\u2010fold\u00a0higher\u00a0mean NfL than slow progressors (17.10 vs. 4.64\u2009pg\/mL). NfL showed no correlation with King's clinical stage (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.00). GFAP correlated with age but not with progression rate or disease duration; S\u2010100B showed no association with progression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Serum NfL on a commercial ECLIA platform retained its strong correlation with progression rate despite unstructured sampling, replicating SIMOA\u2010based findings at platform\u2010specific values. NfL tracked the rate of decline rather than the accumulated disease state; GFAP and S\u2010100B added no prognostic or disease\u2010specific information.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mus.70397","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:46:36Z","timestamp":1788579996000},"page":"1398-1405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serum Neurofilament Light Chain and\n                    <scp>GFAP<\/scp>\n                    in Amyotrophic Lateral Sclerosis on a Commercial\n                    <scp>ECLIA<\/scp>\n                    Platform"],"prefix":"10.1002","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3321-7064","authenticated-orcid":false,"given":"Nicholas S.","family":"Streicher","sequence":"first","affiliation":[{"name":"Department of Neurology Georgetown University  Washington DC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2018-320106"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-018-0058-z"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000001642"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2016.5398"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000009559"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2018-318704"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000004761"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.679199"},{"key":"e_1_2_12_10_1","unstructured":"U.S. Food and Drug Administration \u201cFDA Approves Treatment of Amyotrophic Lateral Sclerosis Associated With a Mutation in the SOD1 Gene \u201d(2023) fda.gov."},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2003715"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2204705"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(24)00216-3"},{"issue":"1","key":"e_1_2_12_14_1","first-page":"12","article-title":"Neurofilaments in the Diagnosis of Motoneuron Diseases: A Prospective Study on 455 Patients","volume":"87","author":"Steinacker P.","year":"2016","journal-title":"Journal of Neurology, Neurosurgery, and 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Nerve"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.70397","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mus.70397","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mus.70397","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:43:10Z","timestamp":1791391390000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mus.70397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/mus.70397"],"URL":"https:\/\/doi.org\/10.1002\/mus.70397","archive":["Portico"],"relation":{},"ISSN":["0148-639X","1097-4598"],"issn-type":[{"value":"0148-639X","type":"print"},{"value":"1097-4598","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]},"assertion":[{"value":"2026-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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