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Intraperitoneal injections of acrylamide (30 mg\/kg\/day, 5 times each week) cause inhibition of staining by this technique so that approximately one third of the end\u2010plates in rat sternocostalis muscle are unstained after 24 hours, and by 17 days more than 70% are unstained. This is not associated with nerve fiber degeneration. A similar inhibition of staining can also be shown after prior incubation of the sternocostalis muscle in 4 m<jats:italic>M<\/jats:italic> acrylamide in oxygenated Ringer's solution. Intraperitoneal injection of the thiol group blocker N\u2010ethylmaleimide also causes marked inhibition of staining of motor end\u2010plates by this method. Dithiothreitol, which prevents the oxidation of thiol groups, will partly prevent the inhibition of staining by both acrylamide and N\u2010ethylmaleimide, when given in vivo.<\/jats:p>","DOI":"10.1002\/mus.880070203","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T04:43:16Z","timestamp":1104554596000},"page":"94-100","source":"Crossref","is-referenced-by-count":15,"title":["Effects of acrylamide and other sulfhydryl compounds in vivo and in vitro on staining of motor nerve terminals by the zinc iodide\u2010osmium technique"],"prefix":"10.1002","volume":"7","author":[{"given":"Sheila","family":"Kemplay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John B.","family":"Cavanagh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(68)90104-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.44.9.848"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1982.tb00300.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687383"},{"key":"e_1_2_1_6_2","first-page":"323","article-title":"Granules et substances r\u00e9duisant l'iodure d'osmium","volume":"4","author":"Champy C","year":"1918","journal-title":"J Anat Physiol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(75)90336-6"},{"key":"e_1_2_1_8_2","first-page":"210","article-title":"Peripheral neuropathy in rats produced by acrylamide","volume":"23","author":"Fullerton PM","year":"1966","journal-title":"Br J Med"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00644545"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480080407"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(70)90009-2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233965"},{"key":"e_1_2_1_13_2","first-page":"180","article-title":"Effects of acrylamide on the central nervous system of the cat","volume":"123","author":"Kuperman AS","year":"1958","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(76)90313-7"},{"key":"e_1_2_1_15_2","first-page":"1","article-title":"La technique de Champy \u00e0 l'osmium\u2010iodure de potassium et la modification de Maillet \u00e0 l'osmium\u2010iodure de zinc","volume":"54","author":"Maillet M","year":"1962","journal-title":"Trab Inst Cajal"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/97.1.235"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.43.1.80"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(75)90222-X"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01938497"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00364309"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-196910000-00004"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.78.3.839"},{"key":"e_1_2_1_23_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1017\/S0317167100019739","article-title":"A review of acrylamide neurotoxicity. 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