{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:02:07Z","timestamp":1760644927666},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T00:00:00Z","timestamp":1097625600000},"content-version":"vor","delay-in-days":6982,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Muscle and Nerve"],"published-print":{"date-parts":[[1985,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conditions have been developed for the culture of rat spinal cord neurons in serum\u2010free media supplemented with hormones and growth factors. Neurons were identified by immunofluorescence\u2010labeled antineurofilament antibody, and their growth was monitored by assay of choline acetyltransferase and cholinesterase activities. Activities of these enzymes were considerably higher than those of comparable cultures in serum supplemented media in which there were visibly many more nonneuronal cells. Serum immunoglobulins from patients with motor neuron disease showed enhanced binding to rat spinal cord cells maintained in both serum\u2010supplemented and serum\u2010free media, as compared with those from normal healthy individuals. Enhanced binding was more marked with the latter cells, presumably because of the higher proportion of neuronal cells in these cultures. Serum immunoglobulins from patients with other neurologic disorders showed a similar binding to that of the normal controls. The results demonstrate the presence of an immune response to spinal cord cell membrane components in patients with motor neuron disease, although whether the response is primary or secondary in the disease process remains unclear.<\/jats:p>","DOI":"10.1002\/mus.880080709","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T08:20:50Z","timestamp":1104567650000},"page":"595-605","source":"Crossref","is-referenced-by-count":23,"title":["Cultured rat spinal cord neurons: Interaction with motor neuron disease immunoglobulins"],"prefix":"10.1002","volume":"8","author":[{"given":"John","family":"Digby","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed","family":"Jehanli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George G.","family":"Lunt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Clifford\u2010Rose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(79)90302-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(79)90387-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.77.1.83"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1965.tb20212.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.1.514"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90202-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)80160-9"},{"key":"e_1_2_1_9_2","volume-title":"Research Progress in Motor Neurone Disease","author":"Clifford\u2010Rose F","year":"1984"},{"key":"e_1_2_1_10_2","first-page":"324","volume-title":"Basic and Clinical Aspects of Molecular Neurobiology","author":"Digby J","year":"1983"},{"key":"e_1_2_1_11_2","unstructured":"DigbyJHarrisonRJehanliALuntGGBehanPO:Motor neurone disease: binding of serum immunoglobulins to rat spinal cord neurones in culture. Abstract Fifth International Congress on Neuromuscular Diseases Marseilles 1982."},{"key":"e_1_2_1_12_2","first-page":"368","volume-title":"Research Progress in Motor Neurone Disease","author":"Digby J","year":"1984"},{"key":"e_1_2_1_13_2","first-page":"249","volume-title":"Research Progress in Motor Neurone Disease","author":"Ecob MS","year":"1984"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00965492"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(61)90145-9"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000112747"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.5475.1399"},{"key":"e_1_2_1_18_2","first-page":"179","volume-title":"Motor Neurone Disease","author":"Field EJM","year":"1969"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(75)90679-4"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1982.tb03771.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(66)90047-9"},{"key":"e_1_2_1_22_2","first-page":"26","article-title":"Serum immunoglobulins from patients with motor neurone disease bind to spinal cord neurones in culture","author":"Harrison R","year":"1982","journal-title":"J Neuroimmunol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1974.00490340060015"},{"key":"e_1_2_1_24_2","first-page":"14.1","volume-title":"Handbook of Experimental Immunology","author":"Hunter WM","year":"1978"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(82)90133-6"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1975.00490450043005"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689566"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measured with the folin phenol reagent","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1159\/000112754"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(74)90139-0"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90399-6"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00736042"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(79)90056-9"},{"key":"e_1_2_1_34_2","first-page":"96","volume-title":"Handbook of Physiology: The Nervous System, 1","author":"Nelson PG","year":"1978"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1977.40.5.1132"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880050109"},{"key":"e_1_2_1_37_2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1523\/JNEUROSCI.01-02-00204.1981","article-title":"Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron\u2010enriched fractions","volume":"11","author":"Schnaar RL","year":"1981","journal-title":"J Neurosci"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1159\/000112485"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6823568"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1180703"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.33.3.317"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00964141"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-008301-5.50016-9"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1977.tb10727.x"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.179.4073.579"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01114913"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.1.142"}],"container-title":["Muscle &amp; 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